Natural Selection and Adaptive Evolution of Leptin in the Ochotona Family Driven by the Cold Environmental Stress

Jie Yang; Zhen Long Wang; Xin Quan Zhao; De Peng Wang; De Lin Qi; Bao Hong Xu; Yong Hong Ren; Hui Fang Tian

doi:10.1371/journal.pone.0001472

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Peer-reviewed

Research Article

Natural Selection and Adaptive Evolution of Leptin in the Ochotona Family Driven by the Cold Environmental Stress

Jie Yang,

Affiliations Key Laboratory of Qinghai-Tibetan Plateau Biological Evolution and Adaptation, Northwest Institute of Plateau Biology, The Chinese Academy of Sciences, Xining, Qinghai, China, Graduate School of the Chinese Academy of Sciences, Beijing, China
⨯
Zhen Long Wang,

Affiliations Key Laboratory of Qinghai-Tibetan Plateau Biological Evolution and Adaptation, Northwest Institute of Plateau Biology, The Chinese Academy of Sciences, Xining, Qinghai, China, College of Life Science, Qufu Normal University, Qufu, Shandong, China
⨯
Xin Quan Zhao ,

*To whom correspondence should be addressed. E-mail: xqzhao@nwipb.ac.cn

Affiliation Key Laboratory of Qinghai-Tibetan Plateau Biological Evolution and Adaptation, Northwest Institute of Plateau Biology, The Chinese Academy of Sciences, Xining, Qinghai, China
⨯
De Peng Wang,

Affiliations Key Laboratory of Qinghai-Tibetan Plateau Biological Evolution and Adaptation, Northwest Institute of Plateau Biology, The Chinese Academy of Sciences, Xining, Qinghai, China, Graduate School of the Chinese Academy of Sciences, Beijing, China
⨯
De Lin Qi,

Affiliations Key Laboratory of Qinghai-Tibetan Plateau Biological Evolution and Adaptation, Northwest Institute of Plateau Biology, The Chinese Academy of Sciences, Xining, Qinghai, China, Graduate School of the Chinese Academy of Sciences, Beijing, China
⨯
Bao Hong Xu,

Affiliation Microbiology Department, Shijiazhuang Center for Disease Control and Prevention, Shijiazhuang, Hebei, China
⨯
Yong Hong Ren,

Affiliation Microbiology Department, Shijiazhuang Center for Disease Control and Prevention, Shijiazhuang, Hebei, China
⨯
Hui Fang Tian

Affiliation Microbiology Department, Shijiazhuang Center for Disease Control and Prevention, Shijiazhuang, Hebei, China
⨯

Natural Selection and Adaptive Evolution of Leptin in the Ochotona Family Driven by the Cold Environmental Stress

Jie Yang,
Zhen Long Wang,
Xin Quan Zhao,
De Peng Wang,
De Lin Qi,
Bao Hong Xu,
Yong Hong Ren,
Hui Fang Tian

Published: January 23, 2008
https://doi.org/10.1371/journal.pone.0001472

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PCR cycling conditions

Posted by phenry on 14 Oct 2009 at 22:04 GMT

PKLEPFOR (forward primer: 5′-aggaaggaaaatgcggtg-3′) and PKLEPREV (reverse primer: 5′- tggaggagtaaaagagaaatgg-3′).
http://plosone.org/article/info:doi/10.1371/journal.pone.0001472#article1.body1.sec4.sec2.p1

Dear colleagues,

Firstly I would like to say that I very much enjoyed reading your article. I am planning on testing these primers on the American pika (O. princeps) and was wondering what were the cycling parameters you used in your PCR reactions.

Thanks in advance for your response,

Sincerely,

Philippe Henry

No competing interests declared.

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Natural Selection and Adaptive Evolution of Leptin in the Ochotona Family Driven by the Cold Environmental Stress

Natural Selection and Adaptive Evolution of Leptin in the Ochotona Family Driven by the Cold Environmental Stress

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PCR cycling conditions

Posted by phenry on 14 Oct 2009 at 22:04 GMT