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PCR cycling conditions

Posted by phenry on 14 Oct 2009 at 22:04 GMT

PKLEPFOR (forward primer: 5′-aggaaggaaaatgcggtg-3′) and PKLEPREV (reverse primer: 5′- tggaggagtaaaagagaaatgg-3′).
http://plosone.org/article/info:doi/10.1371/journal.pone.0001472#article1.body1.sec4.sec2.p1

Dear colleagues,

Firstly I would like to say that I very much enjoyed reading your article. I am planning on testing these primers on the American pika (O. princeps) and was wondering what were the cycling parameters you used in your PCR reactions.

Thanks in advance for your response,

Sincerely,

Philippe Henry

No competing interests declared.