Sequence Depth, Not PCR Replication, Improves Ecological Inference from Next Generation DNA Sequencing

Dylan P. Smith; Kabir G. Peay

doi:10.1371/journal.pone.0090234

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Open Access

Peer-reviewed

Research Article

Sequence Depth, Not PCR Replication, Improves Ecological Inference from Next Generation DNA Sequencing

Dylan P. Smith,

Affiliation Department of Biology, Stanford University, Stanford, California, United States of America
⨯
Kabir G. Peay

* E-mail: kpeay@stanford.edu

Affiliation Department of Biology, Stanford University, Stanford, California, United States of America
⨯

Sequence Depth, Not PCR Replication, Improves Ecological Inference from Next Generation DNA Sequencing

Dylan P. Smith,
Kabir G. Peay

Published: February 28, 2014
https://doi.org/10.1371/journal.pone.0090234

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Apparent amplicon size (gel)

Posted by cement_head on 22 Feb 2017 at 15:39 GMT

Hello,

Would the authors be kind enough to post the apparent molecular mass of the amplicon band on a DNA agarose gel? The size that would include the target region plus the GOLAY code & Illumina adaptors?

Thanks

No competing interests declared.

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Polymerase chain reaction
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Community structure
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Community ecology
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Next-generation sequencing
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Soil ecology
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DNA replication
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Microbial ecology
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Sequence Depth, Not PCR Replication, Improves Ecological Inference from Next Generation DNA Sequencing

Sequence Depth, Not PCR Replication, Improves Ecological Inference from Next Generation DNA Sequencing

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Apparent amplicon size (gel)

Posted by cement_head on 22 Feb 2017 at 15:39 GMT