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closeDiversity of "Lactobacillus" based on culture
Posted by irnewton on 20 Mar 2012 at 15:18 GMT
distance
http://plosone.org/article/info:doi/10.1371/journal.pone.0033188#pone-0033188-g001
This is a really great diversity of LABs in your cultured isolates. It would be good to compare the diversity you find here to that found based on culture-independent approaches to measuring diversity.
RE: Diversity of "Lactobacillus" based on culture
ACVM replied to irnewton on 20 Mar 2012 at 18:38 GMT
Thank you for your comment. We have already looked into the diversity based on culture independent methods by cloning and 16SrDNA sequencing. The diversity of the LAB species and strains depend on changes due to seasons, bee health, presence of other microbes and other conditions as shown in our prevoius publications that you can find in the reference part. But the 16SrDNA is not always a good taxonomy or phylogeny tool any longer and just by looking into a tiny part of the 16SrDNA as some researchers do will not give you any good bacterial diversity anyway even if you use a culture independet method. You need to use other genes for the classification of LAB.