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closePrevious data reported by Paul Yaswen contradicts with current paper
Posted by MikeSh on 10 Apr 2010 at 16:07 GMT
I have one question. The abstract from a report by Paul Yaswen (Functional Analysis of BORIS, A Novel DNA Binding Protein, 2006, available at http://oai.dtic.mil/oai/o...) tells that "Analyses of 17 breast tumor cell lines and of 148 randomly selected primary breast cancer samples (obtained from A. Lindblom, Karolinska Hospital, Sweden) without CTCF mutations (A. Lindblom, unpublished) demonstrated frequencies of BORIS expression of 80 and 88%, respectively. In contrast, using an extremely sensitive 2 step multiplex RT-PCR method, the same team was unable to detect BORIS mRNA in any normal mammary tissues, or other normal somatic tissues (data not shown)". How could the author explain the fact that his previously reported data (obtained from an even larger sample than one used in current paper) completely contradicts with data from his current paper?
RE: Previous data reported by Paul Yaswen contradicts with current paper
PYaswen replied to MikeSh on 10 Apr 2010 at 19:46 GMT
The abstract referred to preliminary data supplied to us by our collaborator. We later found in our own lab that the endpoint RT-PCR method used in these initial studies was unreliable and non-quantitative. In the work reported in the current paper, a quantitative RT-PCR methodology was used. This latter work showed conclusively that the levels of BORIS mRNA in most breast tumor cell lines and tissues were very low or undetectable (< 1 transcript per cell), while CTCF mRNA levels were several orders of magnitude higher.