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closeLifespan extension of class 1 daf-2 mutants by DR
Posted by willmair on 10 Dec 2007 at 20:43 GMT
Surprisingly, DR had no effect on lifespan of daf-2(e1368) adults (Fig. 5A). This finding suggests that DR and daf-2(e1368) may extend adult lifespan through overlapping mechanisms that are not additive.
http://plosone.org/article/info:doi/10.1371/journal.pone.0001240#article1.body1.sec2.sec5.p1
The level of dietary restriction that maximizes lifespan varies for different genotypes. Therefore, before the conclusion that DR does not extend the lifespan of class 1 mutants can be drawn, it would be beneficial to test the lifespan of the e1368 worms across a range of bacterial dilutions (or even dietary deprivation). It may be that although DR via eat-2 mutation does not extend the lifespan of these worms, their lifespan can be increased by DR if the food intake level is optimized.
RE: Lifespan extension of class 1 daf-2 mutants by DR
cwolkow replied to willmair on 12 Dec 2007 at 20:33 GMT
Yes, you have raised a good point. It is difficult to know whether the conditions are optimized for DR, or for IIS reduction for that matter. However, the finding that these daf-2 alleles differed in their responses to eat-2 induced DR is important evidence for some sort of interaction between these pathways.