Elevated PLA2G7 Gene Promoter Methylation as a Gender-Specific Marker of Aging Increases the Risk of Coronary Heart Disease in Females

Danjie Jiang; Dawei Zheng; Lingyan Wang; Yi Huang; Haibo Liu; Leiting Xu; Qi Liao; Panpan Liu; Xinbao Shi; Zhaoyang Wang; Lebo Sun; Qingyun Zhou; Ni Li; Limin Xu; Yanping Le; Meng Ye; Guofeng Shao; Shiwei Duan

doi:10.1371/journal.pone.0059752

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Peer-reviewed

Research Article

Elevated PLA2G7 Gene Promoter Methylation as a Gender-Specific Marker of Aging Increases the Risk of Coronary Heart Disease in Females

Danjie Jiang ,

Contributed equally to this work with: Danjie Jiang, Dawei Zheng, Lingyan Wang

Affiliations Ningbo Medical Center Lihuili Hospital, Ningbo University, Ningbo, Zhejiang, China, The Affiliated Hospital, Ningbo University, Ningbo, Zhejiang, China, Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo University, Ningbo, Zhejiang, China
⨯
Dawei Zheng ,

Contributed equally to this work with: Danjie Jiang, Dawei Zheng, Lingyan Wang

Affiliation Ningbo Medical Center Lihuili Hospital, Ningbo University, Ningbo, Zhejiang, China
⨯
Lingyan Wang ,

Contributed equally to this work with: Danjie Jiang, Dawei Zheng, Lingyan Wang

Affiliation Bank of Blood Products, Ningbo No.2 Hospital, Ningbo, Zhejiang, China
⨯
Yi Huang,

Affiliations The Affiliated Hospital, Ningbo University, Ningbo, Zhejiang, China, Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo University, Ningbo, Zhejiang, China
⨯
Haibo Liu,

Affiliation Yinzhou People’s Hospital, Ningbo University, Ningbo, Zhejiang, China
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Leiting Xu,

Affiliations The Affiliated Hospital, Ningbo University, Ningbo, Zhejiang, China, Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo University, Ningbo, Zhejiang, China
⨯
Qi Liao,

Affiliations The Affiliated Hospital, Ningbo University, Ningbo, Zhejiang, China, Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo University, Ningbo, Zhejiang, China
⨯
Panpan Liu,

Affiliations The Affiliated Hospital, Ningbo University, Ningbo, Zhejiang, China, Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo University, Ningbo, Zhejiang, China
⨯
Xinbao Shi,

Affiliation Ningbo Medical Center Lihuili Hospital, Ningbo University, Ningbo, Zhejiang, China
⨯
Zhaoyang Wang,

Affiliation Ningbo Medical Center Lihuili Hospital, Ningbo University, Ningbo, Zhejiang, China
⨯
Lebo Sun,

Affiliation Ningbo Medical Center Lihuili Hospital, Ningbo University, Ningbo, Zhejiang, China
⨯
Qingyun Zhou,

Affiliation Ningbo Medical Center Lihuili Hospital, Ningbo University, Ningbo, Zhejiang, China
⨯
Ni Li,

Affiliation Ningbo Medical Center Lihuili Hospital, Ningbo University, Ningbo, Zhejiang, China
⨯
Limin Xu,

Affiliations The Affiliated Hospital, Ningbo University, Ningbo, Zhejiang, China, Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo University, Ningbo, Zhejiang, China
⨯
Yanping Le,

Affiliations The Affiliated Hospital, Ningbo University, Ningbo, Zhejiang, China, Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo University, Ningbo, Zhejiang, China
⨯
Meng Ye ,

* E-mail: duanshiwei@nbu.edu.cn (SD); sgf1958@sina.com (GS); dryemeng@yahoo.com.cn (MY)

Affiliation The Affiliated Hospital, Ningbo University, Ningbo, Zhejiang, China
⨯
Guofeng Shao ,

* E-mail: duanshiwei@nbu.edu.cn (SD); sgf1958@sina.com (GS); dryemeng@yahoo.com.cn (MY)

Affiliation Ningbo Medical Center Lihuili Hospital, Ningbo University, Ningbo, Zhejiang, China
⨯
[ ... ],
Shiwei Duan

* E-mail: duanshiwei@nbu.edu.cn (SD); sgf1958@sina.com (GS); dryemeng@yahoo.com.cn (MY)

Affiliations The Affiliated Hospital, Ningbo University, Ningbo, Zhejiang, China, Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo University, Ningbo, Zhejiang, China
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Elevated PLA2G7 Gene Promoter Methylation as a Gender-Specific Marker of Aging Increases the Risk of Coronary Heart Disease in Females

Danjie Jiang,
Dawei Zheng,
Lingyan Wang,
Yi Huang,
Haibo Liu,
Leiting Xu,
Qi Liao,
Panpan Liu,
Xinbao Shi,
Zhaoyang Wang

Published: March 28, 2013
https://doi.org/10.1371/journal.pone.0059752

Reader Comments

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Correlation methylation and expression

Posted by hoffm on 04 Jun 2013 at 13:26 GMT

I wonder why the authors did not measure LpPLA2 activity or mass in their samples and correlate the data with the methylation pattern? As pointed out in the limitation section the authors worked with a mixture of cells and to my knowledge most of them will never secrete LpPLA2. Perhaps they should repeat the work with isolated monocytes.
At this point I miss the functional connection between the methylation of this part of the promoter and CHD.

No competing interests declared.

RE: Correlation methylation and expression

Shiwei1 replied to hoffm on 04 Jun 2013 at 14:24 GMT

Thank you for your comments.
The purpose of this pilot study is to test whether the promoter methylation as a biomarker works in the prediction of the risk of CHD by using the peripheral blood. And you are right about using the monocytes to explore the functional link between methylation and CHD. And it will be even better adding the LpPLA2 actiivity in the serum. We thank you very much for your great suggestion, and will try our best to collect all-angle data in the coming projects.
Best regards,

Shiwei Duan
Ningbo University

No competing interests declared.

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Elevated PLA2G7 Gene Promoter Methylation as a Gender-Specific Marker of Aging Increases the Risk of Coronary Heart Disease in Females

Elevated PLA2G7 Gene Promoter Methylation as a Gender-Specific Marker of Aging Increases the Risk of Coronary Heart Disease in Females

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Correlation methylation and expression

Posted by hoffm on 04 Jun 2013 at 13:26 GMT

RE: Correlation methylation and expression

Shiwei1 replied to hoffm on 04 Jun 2013 at 14:24 GMT