A New Safety Concern for Glaucoma Treatment Demonstrated by Mass Spectrometry Imaging of Benzalkonium Chloride Distribution in the Eye, an Experimental Study in Rabbits

Françoise Brignole-Baudouin; Nicolas Desbenoit; Gregory Hamm; Hong Liang; Jean-Pierre Both; Alain Brunelle; Isabelle Fournier; Vincent Guerineau; Raphael Legouffe; Jonathan Stauber; David Touboul; Maxence Wisztorski; Michel Salzet; Olivier Laprevote; Christophe Baudouin

doi:10.1371/journal.pone.0050180

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Research Article

A New Safety Concern for Glaucoma Treatment Demonstrated by Mass Spectrometry Imaging of Benzalkonium Chloride Distribution in the Eye, an Experimental Study in Rabbits

Françoise Brignole-Baudouin ,

* E-mail: francoise.brignole@inserm.fr

Affiliations INSERM, U968, Paris, France, UPMC Univ Paris 06, UMR_S 968, Institut de la Vision, Paris, France, CNRS, UMR_7210, Paris, France, Centre Hospitalier National d’Ophtalmologie des Quinze-Vingts, INSERM-DHOS CIC 503, Paris, France, Chimie Toxicologie Analytique et Cellulaire, EA 4463, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes, Paris, France
⨯
Nicolas Desbenoit,

Affiliations INSERM, U968, Paris, France, UPMC Univ Paris 06, UMR_S 968, Institut de la Vision, Paris, France, CNRS, UMR_7210, Paris, France, Centre de recherche de Gif, Institut de Chimie des Substances Naturelles, CNRS, Gif-sur-Yvette, France
⨯
Gregory Hamm,

Affiliation Imabiotech Campus Cité Scientifique, Villeneuve d’Ascq, France
⨯
Hong Liang,

Affiliations INSERM, U968, Paris, France, UPMC Univ Paris 06, UMR_S 968, Institut de la Vision, Paris, France, CNRS, UMR_7210, Paris, France, Centre Hospitalier National d’Ophtalmologie des Quinze-Vingts, INSERM-DHOS CIC 503, Paris, France
⨯
Jean-Pierre Both,

Affiliation Laboratoire d’Intégration des Systèmes et des Technologies, CEA-LIST, Gif-sur-Yvette, France
⨯
Alain Brunelle,

Affiliation Centre de recherche de Gif, Institut de Chimie des Substances Naturelles, CNRS, Gif-sur-Yvette, France
⨯
Isabelle Fournier,

Affiliation Laboratoire de Spectrométrie de Masse Biologique, Fondamentale et Appliquée, EA 4550, Université Lille Nord de France – Université Lille 1, Villeneuve d’Ascq, France
⨯
Vincent Guerineau,

Affiliation Centre de recherche de Gif, Institut de Chimie des Substances Naturelles, CNRS, Gif-sur-Yvette, France
⨯
Raphael Legouffe,

Affiliation Imabiotech Campus Cité Scientifique, Villeneuve d’Ascq, France
⨯
Jonathan Stauber,

Affiliation Imabiotech Campus Cité Scientifique, Villeneuve d’Ascq, France
⨯
David Touboul,

Affiliation Centre de recherche de Gif, Institut de Chimie des Substances Naturelles, CNRS, Gif-sur-Yvette, France
⨯
Maxence Wisztorski,

Affiliation Laboratoire de Spectrométrie de Masse Biologique, Fondamentale et Appliquée, EA 4550, Université Lille Nord de France – Université Lille 1, Villeneuve d’Ascq, France
⨯
Michel Salzet,

Affiliation Laboratoire de Spectrométrie de Masse Biologique, Fondamentale et Appliquée, EA 4550, Université Lille Nord de France – Université Lille 1, Villeneuve d’Ascq, France
⨯
Olivier Laprevote,

Affiliation Chimie Toxicologie Analytique et Cellulaire, EA 4463, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes, Paris, France
⨯
Christophe Baudouin

Affiliations INSERM, U968, Paris, France, UPMC Univ Paris 06, UMR_S 968, Institut de la Vision, Paris, France, CNRS, UMR_7210, Paris, France, Centre Hospitalier National d’Ophtalmologie des Quinze-Vingts, INSERM-DHOS CIC 503, Paris, France, Université Versailles Saint-Quentin-en-Yvelines, Versailles, France, Assistance Publique - Hôpitaux de Paris Hôpital Ambroise Paré, Service d’Ophtalmologie, Boulogne-Billancourt, France
⨯

A New Safety Concern for Glaucoma Treatment Demonstrated by Mass Spectrometry Imaging of Benzalkonium Chloride Distribution in the Eye, an Experimental Study in Rabbits

Françoise Brignole-Baudouin,
Nicolas Desbenoit,
Gregory Hamm,
Hong Liang,
Jean-Pierre Both,
Alain Brunelle,
Isabelle Fournier,
Vincent Guerineau,
Raphael Legouffe,
Jonathan Stauber

Published: November 27, 2012
https://doi.org/10.1371/journal.pone.0050180

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shift in figures

Posted by BRIGNOLE on 02 Dec 2012 at 10:25 GMT

MALDI-TOF imaging of whole eye section of a rabbit instilled twice a day with one drop of 0.01% benzalkonium chloride (BAK) for 5 months.
MALDI-TOF imaging shows the BAK distribution in a BAK-treated eye. (a) Histology image of an adjacent cryosection stained with hematoxylin-eosin (HE) showing three areas of interest: cornea (area 1), nasal iridocorneal angle (area 2) and optic nerve area (area 3). (b, c) Overlays between HE staining and MALDI-TOF ion images of BAK C12 and C14 distributions in whole eye section at m/z 304.30 and 332.33, respectively. (d, e) MALDI-TOF ion images of BAK C12 and C14 distributions at m/z 304.30 and 332.33, respectively. Intensities of the ions are represented in colour, based on the intensity scale provided (from black to white). Field of view 16×15 mm. (f, g, h) MALDI-TOF mass spectra extracted from areas 1, 2 and 3, respectively, showing BAK C12 and C14 ion peaks.
http://plosone.org/article/info:doi/10.1371/journal.pone.0050180#pone-0050180-g003

This figure refers to the previous legend, and this legend refers to the next figure

No competing interests declared.

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A New Safety Concern for Glaucoma Treatment Demonstrated by Mass Spectrometry Imaging of Benzalkonium Chloride Distribution in the Eye, an Experimental Study in Rabbits

A New Safety Concern for Glaucoma Treatment Demonstrated by Mass Spectrometry Imaging of Benzalkonium Chloride Distribution in the Eye, an Experimental Study in Rabbits

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shift in figures

Posted by BRIGNOLE on 02 Dec 2012 at 10:25 GMT