Practical Recommendations for the Use of the GeneSwitch Gal4 System to Knock-Down Genes in Drosophila melanogaster

Filippo Scialo; Ashwin Sriram; Rhoda Stefanatos; Alberto Sanz

doi:10.1371/journal.pone.0161817

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Research Article

Practical Recommendations for the Use of the GeneSwitch Gal4 System to Knock-Down Genes in Drosophila melanogaster

Filippo Scialo,

Affiliation Institute for Cell and Molecular Biosciences, Campus for Ageing and Vitality, University of Newcastle, Newcastle-upon-Tyne, NE4 5PL, United Kingdom
⨯
Ashwin Sriram,

Affiliation Institute for Cell and Molecular Biosciences, Campus for Ageing and Vitality, University of Newcastle, Newcastle-upon-Tyne, NE4 5PL, United Kingdom
⨯
Rhoda Stefanatos,

Affiliation Institute for Cell and Molecular Biosciences, Campus for Ageing and Vitality, University of Newcastle, Newcastle-upon-Tyne, NE4 5PL, United Kingdom
⨯
Alberto Sanz

* E-mail: Alberto.Sanz@ncl.ac.uk

Affiliation Institute for Cell and Molecular Biosciences, Campus for Ageing and Vitality, University of Newcastle, Newcastle-upon-Tyne, NE4 5PL, United Kingdom
⨯

Practical Recommendations for the Use of the GeneSwitch Gal4 System to Knock-Down Genes in Drosophila melanogaster

Filippo Scialo,
Ashwin Sriram,
Rhoda Stefanatos,
Alberto Sanz

Published: August 29, 2016
https://doi.org/10.1371/journal.pone.0161817

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MiMIC degradFP

Posted by hbellen on 04 Sep 2016 at 15:42 GMT

Hello,

As an alternatively that is less onerous and allows one to monitor the loss of the protein as it is tagged with GFP, we use the MiMIC degradFP system. This works well in adults for quite a few genes ( Nagakar Jaiswal et al., 2015, eLife).

Hugo Bellen

No competing interests declared.

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Practical Recommendations for the Use of the GeneSwitch Gal4 System to Knock-Down Genes in Drosophila melanogaster

Practical Recommendations for the Use of the GeneSwitch Gal4 System to Knock-Down Genes in Drosophila melanogaster

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MiMIC degradFP

Posted by hbellen on 04 Sep 2016 at 15:42 GMT