Levels of DNA Methylation Vary at CpG Sites across the BRCA1 Promoter, and Differ According to Triple Negative and “BRCA-Like” Status, in Both Blood and Tumour DNA

Sarah L. Daniels; George J. Burghel; Philip Chambers; Shadi Al-Baba; Daniel D. Connley; Ian W. Brock; Helen E. Cramp; Olena Dotsenko; Octavia Wilks; Lynda Wyld; Simon S. Cross; Angela Cox

doi:10.1371/journal.pone.0160174

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Peer-reviewed

Research Article

Levels of DNA Methylation Vary at CpG Sites across the BRCA1 Promoter, and Differ According to Triple Negative and “BRCA-Like” Status, in Both Blood and Tumour DNA

Sarah L. Daniels ,

* E-mail: sarahdanielsx@gmail.com (SLD); a.cox@sheffield.ac.uk (AC)

Affiliation Academic Unit of Molecular Oncology, Department of Oncology and Metabolism, University of Sheffield, Sheffield Medical School, Beech Hill Road, Sheffield, S10 2RX, United Kingdom
⨯
George J. Burghel,

Current address: The Manchester Centre for Genomic Medicine, St. Mary’s Hospital, Oxford Road, Manchester, M13 9WL, United Kingdom

Affiliation Academic Unit of Molecular Oncology, Department of Oncology and Metabolism, University of Sheffield, Sheffield Medical School, Beech Hill Road, Sheffield, S10 2RX, United Kingdom
⨯
Philip Chambers,

Affiliation Leeds Institute of Cancer and Pathology, Section of Pathology and Tumour Biology, Wellcome Trust Brenner Building, University of Leeds, St. James’s University Hospital, Beckett Street, Leeds, LS9 7TF, United Kingdom
⨯
Shadi Al-Baba,

Current address: Leeds Genetics Laboratory, St. James’s University Hospital, Beckett Street, Leeds, LS9 7TF, United Kingdom

Affiliation Sheffield Diagnostic Genetics Service, Sheffield Children’s NHS Foundation Trust, Western Bank, Sheffield, S10 2TH, United Kingdom
⨯
Daniel D. Connley,

Affiliation Academic Unit of Molecular Oncology, Department of Oncology and Metabolism, University of Sheffield, Sheffield Medical School, Beech Hill Road, Sheffield, S10 2RX, United Kingdom
⨯
Ian W. Brock,

Affiliation Academic Unit of Molecular Oncology, Department of Oncology and Metabolism, University of Sheffield, Sheffield Medical School, Beech Hill Road, Sheffield, S10 2RX, United Kingdom
⨯
Helen E. Cramp,

Affiliation Academic Unit of Molecular Oncology, Department of Oncology and Metabolism, University of Sheffield, Sheffield Medical School, Beech Hill Road, Sheffield, S10 2RX, United Kingdom
⨯
Olena Dotsenko,

Affiliation Department of Histopathology, Royal Hallamshire Hospital, Sheffield Teaching Hospitals NHS Foundation Trust, Glossop Road, Sheffield, S10 2JF, United Kingdom
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Octavia Wilks,

Affiliation Academic Unit of Pathology, Department of Neuroscience, Faculty of Medicine, Dentistry & Health, The University of Sheffield, Beech Hill Road, Sheffield, S10 2RX, United Kingdom
⨯
Lynda Wyld,

Affiliation Academic Unit of Surgical Oncology, University of Sheffield Medical School, Beech Hill Road, Sheffield, S10 2RX, United Kingdom
⨯
Simon S. Cross,

Affiliation Academic Unit of Pathology, Department of Neuroscience, Faculty of Medicine, Dentistry & Health, The University of Sheffield, Beech Hill Road, Sheffield, S10 2RX, United Kingdom
⨯
Angela Cox

* E-mail: sarahdanielsx@gmail.com (SLD); a.cox@sheffield.ac.uk (AC)

Affiliations Academic Unit of Molecular Oncology, Department of Oncology and Metabolism, University of Sheffield, Sheffield Medical School, Beech Hill Road, Sheffield, S10 2RX, United Kingdom, Sheffield Institute for Nucleic Acids (SInFoNiA), University of Sheffield, Western Bank, Sheffield, S10 2TN, United Kingdom
⨯

Levels of DNA Methylation Vary at CpG Sites across the BRCA1 Promoter, and Differ According to Triple Negative and “BRCA-Like” Status, in Both Blood and Tumour DNA

Sarah L. Daniels,
George J. Burghel,
Philip Chambers,
Shadi Al-Baba,
Daniel D. Connley,
Ian W. Brock,
Helen E. Cramp,
Olena Dotsenko,
Octavia Wilks,
Lynda Wyld

Published: July 27, 2016
https://doi.org/10.1371/journal.pone.0160174

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Funding acknowledgement

Posted by Deleted_Account on 10 May 2017 at 14:16 GMT

We would like to thank the Breast Cancer Now Tissue Bank for financial support for sample collection for this project.

No competing interests declared.

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Levels of DNA Methylation Vary at CpG Sites across the BRCA1 Promoter, and Differ According to Triple Negative and “BRCA-Like” Status, in Both Blood and Tumour DNA

Levels of DNA Methylation Vary at CpG Sites across the BRCA1 Promoter, and Differ According to Triple Negative and “BRCA-Like” Status, in Both Blood and Tumour DNA

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Funding acknowledgement

Posted by Deleted_Account on 10 May 2017 at 14:16 GMT