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closeFunction ?
Posted by yvesmatthess on 22 Jan 2010 at 13:35 GMT
Did you observe similar effecs in any other cells?
RE: Function ?
dstupack replied to yvesmatthess on 22 Jan 2010 at 17:29 GMT
Can you be more specific as to what you are asking?
The induction of apoptosis, the induction of differentiation markers, the impact on cell cycle?
For example, monocytes differentiation into macrophage is probably not a good long term model to study proliferation - nor is the skin model we used. In tumors, both SCLC and NB showed the proliferative defect phenotype. In many cells, expression of DEDs promotes apoptosis directly, possibly via an amplification pathway that we reported in Oncogene last year.
RE: RE: Function ?
yvesmatthess replied to dstupack on 25 Jan 2010 at 13:46 GMT
Excuse for the vague question. I mean if you overexpress DEDs in other cancer cell lines (e.g HeLa), did you observe similar mitotic defects like in NB7 cells?
RE: RE: RE: Function ?
dstupack replied to yvesmatthess on 30 Mar 2010 at 17:54 GMT
Yves,
Sorry for the delay in reply - I thought that I would receive an email from the journal when you replied and I never did.
(I don't often check my own papers). Yes, we could observe similar defects in the small cell tumors, but not really in primary tumors - they arrested quickly. As shown in the paper, we did note some twin nuclei in the huvec cells, though.
Best, Dwayne