Gremlin Enhances the Determined Path to Cardiomyogenesis

Daisuke Kami; Ichiro Shiojima; Hatsune Makino; Kenji Matsumoto; Yoriko Takahashi; Ryuga Ishii; Atsuhiko T. Naito; Masashi Toyoda; Hirohisa Saito; Masatoshi Watanabe; Issei Komuro; Akihiro Umezawa

doi:10.1371/journal.pone.0002407

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Open Access

Peer-reviewed

Research Article

Gremlin Enhances the Determined Path to Cardiomyogenesis

Daisuke Kami,

Affiliations Department of Reproductive Biology, National Institute for Child Health and Development, Tokyo, Japan, Laboratory for Medical Engineering, Division of Materials Science and Chemical Engineering, Graduate School of Engineering, Yokohama National University, Yokohama, Japan
⨯
Ichiro Shiojima,

Affiliation Department of Cardiovascular Science and Medicine, Chiba University Graduate School of Medicine, Chiba, Japan
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Hatsune Makino,

Affiliation Department of Reproductive Biology, National Institute for Child Health and Development, Tokyo, Japan
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Kenji Matsumoto,

Affiliation Department of Allergy and Immunology, National Institute for Child Health and Development, Tokyo, Japan
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Yoriko Takahashi,

Affiliation Department of Reproductive Biology, National Institute for Child Health and Development, Tokyo, Japan
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Ryuga Ishii,

Affiliation Department of Reproductive Biology, National Institute for Child Health and Development, Tokyo, Japan
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Atsuhiko T. Naito,

Affiliation Department of Cardiovascular Science and Medicine, Chiba University Graduate School of Medicine, Chiba, Japan
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Masashi Toyoda,

Affiliation Department of Reproductive Biology, National Institute for Child Health and Development, Tokyo, Japan
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Hirohisa Saito,

Affiliation Department of Allergy and Immunology, National Institute for Child Health and Development, Tokyo, Japan
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Masatoshi Watanabe,

Affiliation Laboratory for Medical Engineering, Division of Materials Science and Chemical Engineering, Graduate School of Engineering, Yokohama National University, Yokohama, Japan
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Issei Komuro,

Affiliation Department of Cardiovascular Science and Medicine, Chiba University Graduate School of Medicine, Chiba, Japan
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Akihiro Umezawa

* E-mail: umezawa@1985.jukuin.keio.ac.jp

Affiliation Department of Reproductive Biology, National Institute for Child Health and Development, Tokyo, Japan
⨯

Gremlin Enhances the Determined Path to Cardiomyogenesis

Daisuke Kami,
Ichiro Shiojima,
Hatsune Makino,
Kenji Matsumoto,
Yoriko Takahashi,
Ryuga Ishii,
Atsuhiko T. Naito,
Masashi Toyoda,
Hirohisa Saito,
Masatoshi Watanabe

Published: June 11, 2008
https://doi.org/10.1371/journal.pone.0002407

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The other nominated gene---IGF-BP4---From the author

Posted by aumezawa on 14 Jun 2008 at 09:26 GMT

By using the very strict criteria to nominate genes that promote cardiomyogenesis, we found only two genes as candidates. Grem1 and IGF-BP4 were nominated as candidates. Grem1 indeed accelerated cardiomyogenesis of murine teratocarcinoma cells as published in this manuscript. It has just recently published in Nature (Zhu W, et al., IGFBP-4 is an inhibitor of canonical Wnt signaling required for cardiogenesis) that IGF-BP4 is involved in cardiogenesis. We did not include this information in this manuscript, page 2, Results section, the paragraph “GeneChip and statistical analysis”.

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Gremlin Enhances the Determined Path to Cardiomyogenesis

Gremlin Enhances the Determined Path to Cardiomyogenesis

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The other nominated gene---IGF-BP4---From the author

Posted by aumezawa on 14 Jun 2008 at 09:26 GMT