Reader Comments
Post a new comment on this article
Post Your Discussion Comment
Please follow our guidelines for comments and review our competing interests policy. Comments that do not conform to our guidelines will be promptly removed and the user account disabled. The following must be avoided:
- Remarks that could be interpreted as allegations of misconduct
- Unsupported assertions or statements
- Inflammatory or insulting language
Thank You!
Thank you for taking the time to flag this posting; we review flagged postings on a regular basis.
closeControl cells
Posted by sramdas on 05 Jul 2012 at 21:05 GMT
Were any control non-cancerous cells tested for irregular cell division? Do we know this is merely a property of cancerous cell lines?
RE: Control cells
henrytse replied to sramdas on 06 Jul 2012 at 01:35 GMT
Thank you for the question.
We had tested diploid 3T3s in the device at the high confinement condition (3um gap) with a 130kPa substrate. No observations of successful mitosis were made. The cells would contract to signal the start of mitosis, but arrested at this stage and subsequently died soon after.
The ability of cancer cell lines to undergo multi-daughter divisions spontaneously has been observed in literature. However, the rate at which this device induces the generation of multi-daughter divisions is an order of magnitude above what has been reported for HeLa cells. We believe that the mechanical microenvironment plays a significant role in tumorigenesis whether initiation or advancement, this study motivates additional work to explore the relationship of mechanical environments and the maintenance of cellular processes.