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closeLPS-induced septic shock
Posted by Gimena on 22 Nov 2011 at 22:54 GMT
GM-CSF R−/− mice are more resistant to LPS-mediated septic shock.
http://plosone.org/article/info:doi/10.1371/journal.pone.0023025#article1.front1.article-meta1.abstract1.p1
Khameneh HJ and colleagues reported that GM-CSF receptor -/- mice were resistant to LPS-induced septic shock. The investigators showed 80% survival of GM-CSF receptor -/- mice versus 10% of controls 7 days after injection of 50 µg/g LPS i.p. However, the knock-down of GM-CSF receptor was done on 129S mice, a strain known to be resistant to LPS-induced septic shock (Am J Respir Cell Mol Biol. 2011 Aug;45(2):393-402) due to their inability to produce caspase-11 (Nature. 2011 Oct 16;479(7371):117-21). Since the control group was wild type C57B mice, the results of the experiment could be simply because of different strain-related sensitivities to LPS and not to the lack of the GM-CSF receptor itself. A more suitable approach would have been to include mice of the same background as the control group.
RE: LPS-induced septic shock
Ruedl replied to Gimena on 23 Nov 2011 at 02:14 GMT
Dear Ginema,
For your clarification:
the B6.129S1-Csf2rbtm1Cgb/J (GM-CSF Receptor -/-) which we used in our study was back crossed 10 generations to C57BL/6 mice as indicated in the Jackson Mice home page. (http://jaxmice.jax.org/st...). They also indicate to use C57BL/6 as controls.