Next-Generation Sequencing of Lung Cancer EGFR Exons 18-21 Allows Effective Molecular Diagnosis of Small Routine Samples (Cytology and Biopsy)

Dario de Biase; Michela Visani; Umberto Malapelle; Francesca Simonato; Valentina Cesari; Claudio Bellevicine; Annalisa Pession; Giancarlo Troncone; Ambrogio Fassina; Giovanni Tallini

doi:10.1371/journal.pone.0083607

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Peer-reviewed

Research Article

Next-Generation Sequencing of Lung Cancer EGFR Exons 18-21 Allows Effective Molecular Diagnosis of Small Routine Samples (Cytology and Biopsy)

Dario de Biase,

Affiliation Department of Medicine (DIMES) – Anatomic Pathology Unit, Bellaria Hospital, University of Bologna, Bologna, Italy
⨯
Michela Visani,

Affiliation Department of Pharmacology and Biotechnology (FaBiT), University of Bologna, Bologna, Italy
⨯
Umberto Malapelle,

Affiliation Department of Public Health, University of Naples Federico II, Naples, Italy
⨯
Francesca Simonato,

Affiliation Department of Medicine (DIMED) – Surgical Pathology & Cytopathology Unit, University of Padua, Padua, Italy
⨯
Valentina Cesari,

Affiliations Department of Medicine (DIMES) – Anatomic Pathology Unit, Bellaria Hospital, University of Bologna, Bologna, Italy, Department of Pharmacology and Biotechnology (FaBiT), University of Bologna, Bologna, Italy
⨯
Claudio Bellevicine,

Affiliation Department of Public Health, University of Naples Federico II, Naples, Italy
⨯
Annalisa Pession,

Affiliation Department of Pharmacology and Biotechnology (FaBiT), University of Bologna, Bologna, Italy
⨯
Giancarlo Troncone,

Affiliation Department of Public Health, University of Naples Federico II, Naples, Italy
⨯
Ambrogio Fassina,

Affiliation Department of Medicine (DIMED) – Surgical Pathology & Cytopathology Unit, University of Padua, Padua, Italy
⨯
Giovanni Tallini

* E-mail: giovanni.tallini@unibo.it

Affiliation Department of Medicine (DIMES) – Anatomic Pathology Unit, Bellaria Hospital, University of Bologna, Bologna, Italy
⨯

Next-Generation Sequencing of Lung Cancer EGFR Exons 18-21 Allows Effective Molecular Diagnosis of Small Routine Samples (Cytology and Biopsy)

Dario de Biase,
Michela Visani,
Umberto Malapelle,
Francesca Simonato,
Valentina Cesari,
Claudio Bellevicine,
Annalisa Pession,
Giancarlo Troncone,
Ambrogio Fassina,
Giovanni Tallini

Published: December 23, 2013
https://doi.org/10.1371/journal.pone.0083607

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How is the percentage of false positive cases on deep sequencing?

Posted by jianyu on 31 Dec 2013 at 19:14 GMT

Dear Dr. Tallini and Dr. Biase:

Congratulation for your work. It is a useful tool on patient screening for target therapy. I just have a few comments about the work:
1. What is the percentage of false positive cases from your deep sequencing using NGS? Should you have a positive and negative control or use other methods to confirm your positive result by deep sequencing (5% mutation DNA)?
2. If the percentage of mutant EGFR is very low, is there any different on TKI response from the patients with higher copy number? Based on some clinical data, TKI response is not just relative to the status of EGFR mutation, but also to the copy number of the mutant gene.

Jian

No competing interests declared.

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Next-generation sequencing
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Non-small cell lung cancer
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Lung and intrathoracic tumors
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Next-Generation Sequencing of Lung Cancer EGFR Exons 18-21 Allows Effective Molecular Diagnosis of Small Routine Samples (Cytology and Biopsy)

Next-Generation Sequencing of Lung Cancer EGFR Exons 18-21 Allows Effective Molecular Diagnosis of Small Routine Samples (Cytology and Biopsy)

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How is the percentage of false positive cases on deep sequencing?

Posted by jianyu on 31 Dec 2013 at 19:14 GMT