A diagnostic primer pair to distinguish between wMel and wAlbB Wolbachia infections

Meng-Jia Lau; Ary A. Hoffmann; Nancy M. Endersby-Harshman

doi:10.1371/journal.pone.0257781

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Open Access

Peer-reviewed

Research Article

A diagnostic primer pair to distinguish between wMel and wAlbB Wolbachia infections

Meng-Jia Lau ,

Roles Conceptualization, Formal analysis, Investigation, Methodology, Visualization, Writing – original draft

* E-mail: mengjial2@student.unimelb.edu.au

Affiliation Pest and Environmental Adaptation Research Group, Bio21 Institute and the School of BioSciences, The University of Melbourne, Parkville, Victoria, Australia

https://orcid.org/0000-0003-2943-7022

⨯
Ary A. Hoffmann,

Roles Funding acquisition, Supervision, Writing – review & editing

Affiliation Pest and Environmental Adaptation Research Group, Bio21 Institute and the School of BioSciences, The University of Melbourne, Parkville, Victoria, Australia
⨯
Nancy M. Endersby-Harshman

Roles Conceptualization, Methodology, Supervision, Writing – review & editing

Affiliation Pest and Environmental Adaptation Research Group, Bio21 Institute and the School of BioSciences, The University of Melbourne, Parkville, Victoria, Australia
⨯

A diagnostic primer pair to distinguish between wMel and wAlbB Wolbachia infections

Meng-Jia Lau,
Ary A. Hoffmann,
Nancy M. Endersby-Harshman

Published: September 23, 2021
https://doi.org/10.1371/journal.pone.0257781

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PCR Annealing Temperature

Posted by mlau328 on 07 Oct 2021 at 05:25 GMT

Comment from authors:
We are sorry for a typo error in the second paragraph of the “LightCycler® efficiency test” section, the annealing temperature should be 58°C instead if 53°C. The whole sentence should be "followed by 40 cycles of 95°C for 5 seconds (Ramp Rate = 4.8°C/s), 58°C for 15 seconds (Ramp Rate = 2.5°C/s), and 72°C for 30 seconds (Ramp Rate = 4.8°C/s).”

No competing interests declared.

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A diagnostic primer pair to distinguish between wMel and wAlbB Wolbachia infections

A diagnostic primer pair to distinguish between wMel and wAlbB Wolbachia infections

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PCR Annealing Temperature

Posted by mlau328 on 07 Oct 2021 at 05:25 GMT