Comparative study of protease hydrolysis reaction demonstrating Normalized Peptide Bond Cleavage Frequency and Protease Substrate Broadness Index

Shukun Yu; Janne Bech Thoegersen; Karsten Mathias Kragh

doi:10.1371/journal.pone.0239080

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Open Access

Peer-reviewed

Research Article

Comparative study of protease hydrolysis reaction demonstrating Normalized Peptide Bond Cleavage Frequency and Protease Substrate Broadness Index

Shukun Yu ,

Roles Methodology, Project administration, Resources, Writing – original draft

* E-mail: shukun.yu@dupont.com

Affiliation DuPont Nutrition & Biosciences, Brabrand, Aarhus, Denmark

http://orcid.org/0000-0002-7713-3042

⨯
Janne Bech Thoegersen,

Roles Data curation, Investigation, Methodology, Software

Affiliation DuPont Nutrition & Biosciences, Brabrand, Aarhus, Denmark
⨯
Karsten Mathias Kragh

Roles Project administration, Resources, Supervision

Affiliation DuPont Nutrition & Biosciences, Brabrand, Aarhus, Denmark
⨯

Comparative study of protease hydrolysis reaction demonstrating Normalized Peptide Bond Cleavage Frequency and Protease Substrate Broadness Index

Shukun Yu,
Janne Bech Thoegersen,
Karsten Mathias Kragh

Published: September 21, 2020
https://doi.org/10.1371/journal.pone.0239080

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Word error in Figure 4 title: pancreatin is the right word, not pepsin.

Posted by syu105 on 29 Jul 2021 at 06:43 GMT

Dear Readers,
Figure 4A and 4B deal with pancreatin, so in the title the word pepsin should be pancreatin. Sorry for this error. The two figures in Fig. 4 are correct.

Fig 4. NPBCF of SPI by FNA, porcine pepsin and their combinations. Conditions: pH 6.5, SPI:protease ratio of 20:1
(A), n = 2, and 100:1(B), n = 3.

Thanks, Dr Shukun Yu, Senior staff scientist, IFF (DuPont Nutrition & Biosciences Aps), Edwin Rahrs Vej 38, DK 8220 Brabrand, Aarhus, Denmark.

No competing interests declared.

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Proteases
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Pepsins
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Liquid chromatography-mass spectrometry
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Hydrolysis
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Protein isolation
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Serine proteases
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Comparative study of protease hydrolysis reaction demonstrating Normalized Peptide Bond Cleavage Frequency and Protease Substrate Broadness Index

Comparative study of protease hydrolysis reaction demonstrating Normalized Peptide Bond Cleavage Frequency and Protease Substrate Broadness Index

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Word error in Figure 4 title: pancreatin is the right word, not pepsin.

Posted by syu105 on 29 Jul 2021 at 06:43 GMT