Reader Comments
Post a new comment on this article
Post Your Discussion Comment
Please follow our guidelines for comments and review our competing interests policy. Comments that do not conform to our guidelines will be promptly removed and the user account disabled. The following must be avoided:
- Remarks that could be interpreted as allegations of misconduct
- Unsupported assertions or statements
- Inflammatory or insulting language
Thank You!
Thank you for taking the time to flag this posting; we review flagged postings on a regular basis.
closequantification required
Posted by Joanna on 04 Oct 2012 at 20:49 GMT
bacterial cells diffused into the soft media to some extent (Fig. 2)
http://plosone.org/article/info:doi/10.1371/journal.pone.0046447#article1.body1.sec2.sec2.p1
tube 3 looks much more like tube 2 (no motility) than like tube 1. Turbidity should be measured or more established test on soft agar plates should be used. Data presented here is not convincing.
RE: quantification required
huasongzou replied to Joanna on 08 Nov 2012 at 07:10 GMT
The swimimg ability on soft agar plates were deleted from the MS according to reviewer's suggestion. The restoration is as same weak as in tubes because the Sinorhizobium meliloti has four flagellin genes. It is not supprised to gain a weak restoration phenotype by using the Las flagellin coding gene to complement four flagellin gene deficient mutant in Sinorhizobium meliloti.
RE: RE: quantification required
huasongzou replied to huasongzou on 08 Nov 2012 at 07:37 GMT
Really hope this causal agent can be successfully cultured in lab condition soon. And then the contribution of Las flagellin to motility can be evaluated in own genetic backgroud.