Reviewer #1's Review

“Following a recent experimental work on KaiC oscillations in vitro (ref 12), Sasai and coworkers have proposed a computational model that produces semi-quantitative agreement with these results. The main novelty of their work is the development of the experimentally motivated scheme of KaiC, KaiA, and KaiB allosteric transitions, protein associations, and phosphorilation/dephosphorilation reactions (Fig. 1). In addition, the authors have provided a simple qualitative picture of the oscillatory behavior, based on the catch-and-release mechanism. This paper demonstrates computationally that circadian rhythmicity may arise through processes not requiring transcription-translation. Overall, this is an important contribution, which advances our understanding of the circadian rhythm mechanisms.”

n.b. These are the general comments made by the reviewer when reviewing this paper. Specific points addressed during revision of the paper are not shown.