Erythroid-Specific Expression of β-globin from Sleeping Beauty-Transduced Human Hematopoietic Progenitor Cells

Lucas M. Sjeklocha; Chang-Won Park; Phillip Y-P Wong; Mark J. Roney; John D. Belcher; Dan S. Kaufman; Gregory M. Vercellotti; Robert P. Hebbel; Clifford J. Steer

doi:10.1371/journal.pone.0029110

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Research Article

Erythroid-Specific Expression of β-globin from Sleeping Beauty-Transduced Human Hematopoietic Progenitor Cells

Lucas M. Sjeklocha,

Affiliation Division of Gastroenterology, Hepatology and Nutrition, Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America
⨯
Chang-Won Park,

Affiliation Division of Gastroenterology, Hepatology and Nutrition, Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America
⨯
Phillip Y-P Wong,

Affiliation Division of Gastroenterology, Hepatology and Nutrition, Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America
⨯
Mark J. Roney,

Affiliations Vascular Biology Center, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America, Division of Hematology, Oncology and Transplantation, Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America
⨯
John D. Belcher,

Affiliations Vascular Biology Center, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America, Division of Hematology, Oncology and Transplantation, Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America
⨯
Dan S. Kaufman,

Affiliations Division of Hematology, Oncology and Transplantation, Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America, Stem Cell Institute, University of Minnesota Medical School, University of Minnesota, Minneapolis, Minnesota, United States of America
⨯
Gregory M. Vercellotti,

Affiliations Vascular Biology Center, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America, Division of Hematology, Oncology and Transplantation, Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America
⨯
Robert P. Hebbel,

Affiliations Vascular Biology Center, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America, Division of Hematology, Oncology and Transplantation, Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America
⨯
Clifford J. Steer

* E-mail: steer001@umn.edu

Affiliations Division of Gastroenterology, Hepatology and Nutrition, Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America, Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota, United States of America
⨯

Erythroid-Specific Expression of β-globin from Sleeping Beauty-Transduced Human Hematopoietic Progenitor Cells

Lucas M. Sjeklocha,
Chang-Won Park,
Phillip Y-P Wong,
Mark J. Roney,
John D. Belcher,
Dan S. Kaufman,
Gregory M. Vercellotti,
Robert P. Hebbel,
Clifford J. Steer

Published: December 28, 2011
https://doi.org/10.1371/journal.pone.0029110

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Publisher's Note: Error in Figure 2 legend.

Posted by PLOS_ONE_Group on 30 Jan 2012 at 20:43 GMT

Granulocyte cultures were stained with CD15-PE-Cy7 (left panel); erythrocyte cultures with CD235a-FITC and DRAQ-5 at 1∶2000 (center left panel); B-cell cultures were stained for CD19-AlexaFluor700 (center panel); T-cell cultures with CD1a-FITC and CD7-AlexaFluor700 (center right panel); and myelocyte/monocyte cultures were stained with CD14-V450 and CD33-AlexaFluor700 (right panel).
http://plosone.org/article/info:doi/10.1371/journal.pone.0029110#pone-0029110-g002

There is an error in the legend of Figure 2. The correct text is, “T-cell cultures were stained with CD1a-FITC and CD7-AlexaFluor700 (farleft panel); B-cell cultures were stained for CD19-AlexaFluor700 (centerleft panel); myelocyte/monocyte cultures were stained with CD14-V450 andCD33-AlexaFluor700 (center panel); granulocyte cultures were stained withCD15-PE-Cy7 (right center panel); and erythrocyte cultures with CD235a-FITCand DRAQ-5 at 1:2000 (far right panel).”

No competing interests declared.

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Erythroid-Specific Expression of β-globin from Sleeping Beauty-Transduced Human Hematopoietic Progenitor Cells

Erythroid-Specific Expression of β-globin from Sleeping Beauty-Transduced Human Hematopoietic Progenitor Cells

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Publisher's Note: Error in Figure 2 legend.

Posted by PLOS_ONE_Group on 30 Jan 2012 at 20:43 GMT