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closeDepletion of cohesin promotes silence
Posted by Juan1 on 11 Apr 2007 at 02:04 GMT
I guess that depletion of some mitotic proteins promotes “silence”
It did happen in the past when we showed that depletion of a core unit of the anaphase promoting complex does not block anaphase (Cell Cycle, 4:1561-1575, 2005) and it happens again when we show that depletion of cohesin does not affect cohesion (PLoS ONE. 2: e318, 2007)
The anaphase promoting complex is dispensable for anaphase in mammals and cohesin is dispensable for cohesion in Xenopus and mammals...and 20 days after been published, nobody has anything to say about it (not even the champion of the “free flow of information”).
Separase depletion renders multiple pre-anaphase phenotypes (Cell Cycle, 4:1576-1584, 2005). Proteasome inhibition blocks cells in metaphase in the absence of securin (Cell Cycle, 4:1558-1560, 2005). Activation of the spindle assembly checkpoint blocks mitotic progression in the absence of members of the APC pathway (Cell Cycle, 4:1555-1557, 2005). Cohesin is mantained in the absence of detectable cohesin (PLoS ONE, 1:e53, 2006)....and so on...
Well, up to you. At the end science is what we all make of it...and cells behave their own way, like it or not.
P.S: Of course, this message is my entire responsability: Laura and Duncan are both polite and reasonable people and they would both would refuse posting it (that is also the reason why I don’t quote data from other labs challenging the accepted model).
Cheers
Juan F. Giménez-Abián
RE: Depletion of cohesin promotes silence
Juan1 replied to Juan1 on 11 Apr 2007 at 14:12 GMT
Instead of "cohesin is mantained in the absence of detectable cohesin" it should say "cohesion is maintained in the absence of detectable cohesin" (apologies for my terrible Spanglish!)
...by the way, has anyone tried depleting cohesin subunits and also failed to obtain the expected accumulation of cells in mitosis, with separated sisters?
or...did the cells separate sisters when depleting APC core subunits?
If data challenging a model, or simply not fitting it, remains hidden the model keeps growing in strength, but in the wrong direction
Cheers
Juan F. Giménez-Abián