Cohesin Is Dispensable for Centromere Cohesion in Human Cells

Laura A. Díaz-Martínez; Juan F. Giménez-Abián; Duncan J. Clarke

doi:10.1371/journal.pone.0000318

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Research Article

Cohesin Is Dispensable for Centromere Cohesion in Human Cells

Laura A. Díaz-Martínez ,

Contributed equally to this work with: Laura A. Díaz-Martínez, Juan F. Giménez-Abián

Affiliation Department of Genetics, Cell Biology and Development, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America
⨯
Juan F. Giménez-Abián ,

Contributed equally to this work with: Laura A. Díaz-Martínez, Juan F. Giménez-Abián

Affiliations Department of Genetics, Cell Biology and Development, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America, Proliferación Celular, Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain
⨯
Duncan J. Clarke

*To whom correspondence should be addressed. E-mail: Duncan.J.Clarke-2@umn.edu

Affiliation Department of Genetics, Cell Biology and Development, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America
⨯

Cohesin Is Dispensable for Centromere Cohesion in Human Cells

Laura A. Díaz-Martínez,
Juan F. Giménez-Abián,
Duncan J. Clarke

Published: March 28, 2007
https://doi.org/10.1371/journal.pone.0000318

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Addendum

Posted by LA_Diaz-Martinez on 04 Apr 2007 at 18:18 GMT

siRNA sequences are shown in
http://plosone.org/article/info:doi/10.1371/journal.pone.0000318#article1.body1.sec4.sec3.p1

The siRNA sequence used to knock-down Smc1 is not specified in the original text. Two siRNA sequences targeting Smc1 were used: GCUGCAGCUCUUUAAGCUU(dTdT) and that previously published by Musio, et al., [1]. Although we were unable to confirm the knock-down of Smc1 by western blot, transfection of either siRNA resulted in an identical, strong phenotype. The data included in Figure 4 were obtained using the published siRNA sequence [1].

1. Musio A, Montagna C, Mariani T, Tilenni M, Focarelli ML, et al. (2005) SMC1 involvement in fragile site expression. Hum Mol Genet 14: 525-533.

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Cohesin Is Dispensable for Centromere Cohesion in Human Cells

Cohesin Is Dispensable for Centromere Cohesion in Human Cells

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Addendum

Posted by LA_Diaz-Martinez on 04 Apr 2007 at 18:18 GMT