Dear Authors.
I'm a bit confused about the fold changes presented in your manuscript.
How is the fold change calculated in tables 3,4 and 5? It seems that they are just ratio's of (FPKM-AD/FPKM-N)?
As an example: if i would calculate the fold change for PCK1 in table 4 according to cufflinks (In (FPKM-AD / FPKM-N)/ In (2) ) i would get a fold change of
In (3.186619/0.121441)/ In (2) = 4.71 instead of 26.24.
If my calculations are true, the results would therefore overrepresent much larger fold changes than are actually true.
Can you please clarify this? Thank you in advance
Greetings
Daan van Abel