Reader Comments
Post a new comment on this article
Post Your Discussion Comment
Please follow our guidelines for comments and review our competing interests policy. Comments that do not conform to our guidelines will be promptly removed and the user account disabled. The following must be avoided:
- Remarks that could be interpreted as allegations of misconduct
- Unsupported assertions or statements
- Inflammatory or insulting language
Thank You!
Thank you for taking the time to flag this posting; we review flagged postings on a regular basis.
closeis there a difference in the vector backbones?
Posted by derek_japan on 22 Mar 2007 at 06:13 GMT
Wanted to also ask another quick question. Could there be a link with some sequence or proteins expressed on the vector backbone? This could explain why the effect was not seen in vitro when purified protein was used.
If the vector backbone for EGFP and DsRed had differences to that used for pmaxGFP, this may also explain the different response?
I assume you must have transferred the genes to a different vector as pDsRed from Clontech is a prokaryotic expression vector?
RE: is there a difference in the vector backbones?
mathijs replied to derek_japan on 11 Apr 2007 at 11:04 GMT
pEGFP and pDsRed-N1 (eukaryotic expression vector) have the same backbone. The pmaxGFP sequence is not available, but the amaxa website shows the presence of a CMV promotor, a SV40 pA, a pUC ori and the kanamycin gene. The only expressed feature is kanamycin, also present in the pEGFP and pDsRed-N1 vectors.