Inducible Knockdown of Plasmodium Gene Expression Using the glmS Ribozyme

Parichat Prommana; Chairat Uthaipibull; Chayaphat Wongsombat; Sumalee Kamchonwongpaisan; Yongyuth Yuthavong; Ellen Knuepfer; Anthony A. Holder; Philip J. Shaw

doi:10.1371/journal.pone.0073783

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Open Access

Peer-reviewed

Research Article

Inducible Knockdown of Plasmodium Gene Expression Using the glmS Ribozyme

Parichat Prommana,

Affiliation National Center for Genetic Engineering and Biotechnology (BIOTEC), Khlong Nueng, Khlong Luang, Pathum Thani, Thailand
⨯
Chairat Uthaipibull,

Affiliation National Center for Genetic Engineering and Biotechnology (BIOTEC), Khlong Nueng, Khlong Luang, Pathum Thani, Thailand
⨯
Chayaphat Wongsombat,

Affiliation National Center for Genetic Engineering and Biotechnology (BIOTEC), Khlong Nueng, Khlong Luang, Pathum Thani, Thailand
⨯
Sumalee Kamchonwongpaisan,

Affiliation National Center for Genetic Engineering and Biotechnology (BIOTEC), Khlong Nueng, Khlong Luang, Pathum Thani, Thailand
⨯
Yongyuth Yuthavong,

Affiliation National Center for Genetic Engineering and Biotechnology (BIOTEC), Khlong Nueng, Khlong Luang, Pathum Thani, Thailand
⨯
Ellen Knuepfer,

Affiliation Division of Parasitology, MRC National Institute for Medical Research, London, United Kingdom
⨯
Anthony A. Holder,

Affiliation Division of Parasitology, MRC National Institute for Medical Research, London, United Kingdom
⨯
Philip J. Shaw

* E-mail: philip@biotec.or.th

Affiliation National Center for Genetic Engineering and Biotechnology (BIOTEC), Khlong Nueng, Khlong Luang, Pathum Thani, Thailand
⨯

Inducible Knockdown of Plasmodium Gene Expression Using the glmS Ribozyme

Parichat Prommana,
Chairat Uthaipibull,
Chayaphat Wongsombat,
Sumalee Kamchonwongpaisan,
Yongyuth Yuthavong,
Ellen Knuepfer,
Anthony A. Holder,
Philip J. Shaw

Published: August 30, 2013
https://doi.org/10.1371/journal.pone.0073783

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Information regarding GlcN inducer

Posted by pjs3445 on 21 May 2015 at 01:04 GMT

The authors wish to clarify the source of Glucosamine (GlcN) used as the ribozyme inducer. In all experiments, the inducer compound used was D(+)Glucosamine-HCl >=99% pure tissue culture grade from Sigma, Cat no. G1514.

No competing interests declared.

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Ribozymes
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Plasmodium
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Gene expression
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Red blood cells
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Malarial parasites
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Transfection
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Reporter genes
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Gene targeting
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Inducible Knockdown of Plasmodium Gene Expression Using the glmS Ribozyme

Inducible Knockdown of Plasmodium Gene Expression Using the glmS Ribozyme

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Information regarding GlcN inducer

Posted by pjs3445 on 21 May 2015 at 01:04 GMT