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Texas Red filter

Posted by SteffenDietzel on 15 Dec 2021 at 09:16 GMT

Citation from: Cell Painting, a high-content image-based assay for morphological profiling using multiplexed fluorescent dyes. Mark-Anthony Bray, Shantanu Singh, Han Han, Chadwick T Davis, Blake Borgeson, Cathy Hartland, Maria Kost-Alimova, Sigrun M Gustafsdottir, Christopher C Gibson & Anne E Carpenter. Nature Protocols volume 11, pages 1757–1774 (2016) (Legend to Table 1)

"In Gustafsdottir et al., the TexasRed filter was incorrectly listed as having excitation/emission at 562/642 nm; the actual numbers are 562/624 nm."

No competing interests declared.

RE: Texas Red filter

AnneCarpenter replied to SteffenDietzel on 22 Dec 2021 at 17:05 GMT

Indeed, thank you for pointing out the typo. The microscope's TexasRed filter is listed as excitation/emission (562/642 nm) when it is actually (562/624 nm). This was confirmed with the screening center that performed the microscopy described in the paper.

No competing interests declared.