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closeantibodies for detecting somal iglutamic acid decarboxylase (GAD)
Posted by pnlop1 on 24 Aug 2013 at 19:32 GMT
As the authors infer, there are many antibodies against GAD65 and GAD67 and GABA that greatly vary in their specificity for these molecules. A rabbit antibody call K-2 was good at detecting GABAergic cell bodies (PMID: 1988566 ) but this is no longer commercially available. A mouse monoclonal called K-87 which was made against a peptide specific for GAD67 gives even better at staining GABAergic somata and dendrites and is commercially available, although an antigen-retrival step is necessary. We have used this very successfully on mouse and human tissues, avoiding the in situ hybridization. I hope this helps.
RE: antibodies for detecting somal iglutamic acid decarboxylase (GAD)
Changjiu replied to pnlop1 on 26 Aug 2013 at 19:33 GMT
Thanks for your useful information. We may try this antibody in our future work.