Integrated laboratory protocol for the diagnosis of Sexually Transmitted Infections (STIs): Standardized pre-analytical procedures, rapid screening, hemagglutination, and ELISA methods for use in resource-limited settings

Faramalala Mamitiana Ramamonjiarisoa; Zara Nomentsoa Razafiarimanga; Roseline Ramaroson

doi:10.1371/journal.pone.0346598

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Integrated laboratory protocol for the diagnosis of Sexually Transmitted Infections (STIs): Standardized pre-analytical procedures, rapid screening, hemagglutination, and ELISA methods for use in resource-limited settings

Faramalala Mamitiana Ramamonjiarisoa ,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Writing – original draft, Writing – review & editing

* E-mail: framamonjiarisoa@ymail.com

Affiliation Doctoral School of Life and Environmental Sciences (SVE), Research Team: EAD-7 - Immunology, Immunopathology, Immunodiagnostics, University of Antananarivo, Antananarivo, Madagascar

https://orcid.org/0009-0007-9720-3259

⨯
Zara Nomentsoa Razafiarimanga,

Roles Project administration, Supervision, Validation, Visualization

Affiliation Associate Professor, Director of Documentation and Publications, University of Antananarivo, Antananarivo, Madagascar
⨯
Roseline Ramaroson

Roles Formal analysis, Investigation, Methodology, Project administration, Resources, Validation, Writing – review & editing

Affiliation Faculty of Sciences, University of Antananarivo, Antananarivo, Madagascar
⨯

Integrated laboratory protocol for the diagnosis of Sexually Transmitted Infections (STIs): Standardized pre-analytical procedures, rapid screening, hemagglutination, and ELISA methods for use in resource-limited settings

Faramalala Mamitiana Ramamonjiarisoa,
Zara Nomentsoa Razafiarimanga,
Roseline Ramaroson

Published: May 5, 2026
https://doi.org/10.1371/journal.pone.0346598

About the Authors

Faramalala Mamitiana Ramamonjiarisoa

Roles Conceptualization, Data curation, Formal analysis, Investigation, Writing – original draft, Writing – review & editing

* E-mail: framamonjiarisoa@ymail.com

Affiliation Doctoral School of Life and Environmental Sciences (SVE), Research Team: EAD-7 - Immunology, Immunopathology, Immunodiagnostics, University of Antananarivo, Antananarivo, Madagascar

https://orcid.org/0009-0007-9720-3259

Zara Nomentsoa Razafiarimanga

Roles Project administration, Supervision, Validation, Visualization

Affiliation Associate Professor, Director of Documentation and Publications, University of Antananarivo, Antananarivo, Madagascar

Roseline Ramaroson

Roles Formal analysis, Investigation, Methodology, Project administration, Resources, Validation, Writing – review & editing

Affiliation Faculty of Sciences, University of Antananarivo, Antananarivo, Madagascar

Competing Interests

The authors have declared that no competing interests exist.

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Government laboratories
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Virus testing
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Enzyme-linked immunoassays
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Syphilis
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Chlamydia infection
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Centrifugation
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Sexually transmitted diseases
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Specimen storage
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