About the Authors

Hiroyuki Tomari

Roles Data curation, Investigation, Methodology, Resources, Writing – original draft

☯ These authors contributed equally to this paper

Affiliation IVF Nagata Clinic, Fukuoka-shi, Fukuoka, Japan

Emi Sugizaki

Roles Data curation, Investigation, Resources

☯ These authors contributed equally to this paper

Affiliation Innovative Decontamination Technology Development and Evaluation Center, MIURA CO. LTD., Yokohama-shi, Kanagawa, Japan

Shaimaa Ibrahim

Roles Investigation

Affiliation Innovative Decontamination Technology Development and Evaluation Center, MIURA CO. LTD., Yokohama-shi, Kanagawa, Japan

Yuki Hashiguchi

Roles Investigation

Affiliation Low-Temperature Sterilizer Technical Section, Medical Hospital Equipment Technical Department, MIURA CO. LTD., Matsuyama-shi, Ehime, Japan

Genki Koyama

Roles Resources

Affiliation Innovative Decontamination Technology Development and Evaluation Center, MIURA CO. LTD., Yokohama-shi, Kanagawa, Japan

Yasuo Nakamura

Roles Resources

Affiliation Innovative Decontamination Technology Development and Evaluation Center, MIURA CO. LTD., Yokohama-shi, Kanagawa, Japan

Mayu Nagata

Roles Investigation, Resources

Affiliation IVF Nagata Clinic, Fukuoka-shi, Fukuoka, Japan

Yumi Nagata

Roles Supervision

Affiliation IVF Nagata Clinic, Fukuoka-shi, Fukuoka, Japan

Yuji Haishima

Roles Conceptualization, Methodology, Project administration, Writing – original draft, Writing – review & editing

haishima_yuji@miuraz.co.jp

Affiliations Innovative Decontamination Technology Development and Evaluation Center, MIURA CO. LTD., Yokohama-shi, Kanagawa, Japan, National Institute of Health Sciences, Kawasaki-shi, Kanagawa, Japan

Competing Interests

The authors have declared that no competing interests exist. This study did not receive any specific funding other than financial support in the form of salaries, research materials, and conference participation and travel from the authors’ affiliated institutions. No author received any compensation or honorarium for consulting, speaking engagements, presentations, speaker bureau services, writing, educational events, or expert testimony. MIURA CO., LTD. manufactures and sells ozone/hydroxy peroxide mixed gas sterilizer and the information has been disclosed in the Materials and methods section of the manuscript and on the company website: https://www.miuraz.co.jp/product/industry/xz.html. Yuji Haishima has involved as a co-inventor of the patent ‘P2016-154835A’ titled ‘Decontamination apparatus and method’, and Emi Sugizaki, Shaimaa Ibrahim, Genki Koyama, Yasuo Nakamura, and Yuji Haishima are also co-inventors of the pending patent ‘P2025-71735A, titled ‘Removal method of hydrogen peroxide’, both of which are related intellectual properties to this study. The patent information has also been disclosed on the following websites: https://www.j-platpat.inpit.go.jp/p0200 and https://www.j-platpat.inpit.go.jp/p0200, respectively. None of the authors serve on a Data Safety Monitoring Board or Advisory Board. Yuji Haishima serves as a council member of the Japanese Society for Biomaterials without compensation. None of the authors or their family members hold shares or stock options. No authors received equipment, supplies, pharmaceuticals, medical writing assistance, gifts, or other services from external sources. No authors have other financial or non-financial interests. These disclosures do not alter our adherence to PLOS ONE policies on sharing data and materials.