Determining minimal inhibitory concentrations and antibiotic susceptibility for Enterobacterales by flow cytometry using reactive oxygen species as a marker

Jia Hao Yeo; Nasren Begam; Wan Ting Leow; Andrea Lay-Hoon Kwa

doi:10.1371/journal.pone.0331217

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Open Access

Peer-reviewed

Research Article

Determining minimal inhibitory concentrations and antibiotic susceptibility for Enterobacterales by flow cytometry using reactive oxygen species as a marker

Jia Hao Yeo,

Roles Conceptualization, Data curation, Investigation, Visualization, Writing – original draft

Affiliations Division of Pharmacy, Singapore General Hospital, Singapore, SingHealth-Duke-NUS Academic Clinical Programme, Singapore

https://orcid.org/0000-0001-6478-5888

⨯
Nasren Begam,

Roles Investigation, Project administration

Affiliation Division of Pharmacy, Singapore General Hospital, Singapore
⨯
Wan Ting Leow,

Roles Methodology, Project administration

Affiliation Division of Pharmacy, Singapore General Hospital, Singapore
⨯
Andrea Lay-Hoon Kwa

* E-mail: andrea.kwa.l.h@sgh.com.sg

Affiliations Division of Pharmacy, Singapore General Hospital, Singapore, SingHealth-Duke-NUS Academic Clinical Programme, Singapore, Department of Pharmacy, National University of Singapore, Singapore, Emerging Infection Diseases Program, Duke-NUS Graduate Medical School, Singapore
⨯

Determining minimal inhibitory concentrations and antibiotic susceptibility for Enterobacterales by flow cytometry using reactive oxygen species as a marker

Jia Hao Yeo,
Nasren Begam,
Wan Ting Leow,
Andrea Lay-Hoon Kwa

Published: September 4, 2025
https://doi.org/10.1371/journal.pone.0331217

About the Authors

Jia Hao Yeo

Roles Conceptualization, Data curation, Investigation, Visualization, Writing – original draft

Affiliations Division of Pharmacy, Singapore General Hospital, Singapore, SingHealth-Duke-NUS Academic Clinical Programme, Singapore

https://orcid.org/0000-0001-6478-5888

Nasren Begam

Roles Investigation, Project administration

Affiliation Division of Pharmacy, Singapore General Hospital, Singapore

Wan Ting Leow

Roles Methodology, Project administration

Affiliation Division of Pharmacy, Singapore General Hospital, Singapore

Andrea Lay-Hoon Kwa

* E-mail: andrea.kwa.l.h@sgh.com.sg

Affiliations Division of Pharmacy, Singapore General Hospital, Singapore, SingHealth-Duke-NUS Academic Clinical Programme, Singapore, Department of Pharmacy, National University of Singapore, Singapore, Emerging Infection Diseases Program, Duke-NUS Graduate Medical School, Singapore

Competing Interests

The authors have declared that no competing interests exist.

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Antibiotics
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Flow cytometry
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Bacterial broth dilution
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Reactive oxygen species
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Escherichia coli
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Klebsiella pneumoniae
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Polymyxins
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Singapore
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