Verification of the chromosome number using cytogenetics and estimation of genome size via flow cytometry and k-mer analyses for representative Anoectochilus roxburghii accessions

Wenting Zhang; Yu Mei; Jihua Wang

doi:10.1371/journal.pone.0322457

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Open Access

Peer-reviewed

Research Article

Verification of the chromosome number using cytogenetics and estimation of genome size via flow cytometry and k-mer analyses for representative Anoectochilus roxburghii accessions

Wenting Zhang,

Roles Formal analysis, Writing – original draft, Writing – review & editing

Affiliation Crop Research Institute, Guangdong Academy of Agriculture Sciences/Guangdong Provincial Key Laboratory of Crops Genetics and Improvement Guangdong/Guangdong Provincial Engineering & Technology Research Center for Conservation and Utilization of the Genuine Southern Medicinal Resources, Guangzhou, Guangdong, China

https://orcid.org/0000-0001-7670-5574

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Yu Mei,

Roles Formal analysis, Writing – original draft, Writing – review & editing

Affiliation Crop Research Institute, Guangdong Academy of Agriculture Sciences/Guangdong Provincial Key Laboratory of Crops Genetics and Improvement Guangdong/Guangdong Provincial Engineering & Technology Research Center for Conservation and Utilization of the Genuine Southern Medicinal Resources, Guangzhou, Guangdong, China
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Jihua Wang

Roles Supervision, Writing – original draft, Writing – review & editing

* E-mail: wangjihua@gdaas.cn

Affiliation Crop Research Institute, Guangdong Academy of Agriculture Sciences/Guangdong Provincial Key Laboratory of Crops Genetics and Improvement Guangdong/Guangdong Provincial Engineering & Technology Research Center for Conservation and Utilization of the Genuine Southern Medicinal Resources, Guangzhou, Guangdong, China
⨯

Verification of the chromosome number using cytogenetics and estimation of genome size via flow cytometry and k-mer analyses for representative Anoectochilus roxburghii accessions

Wenting Zhang,
Yu Mei,
Jihua Wang

Published: May 28, 2025
https://doi.org/10.1371/journal.pone.0322457

About the Authors

Wenting Zhang

Roles Formal analysis, Writing – original draft, Writing – review & editing

Affiliation Crop Research Institute, Guangdong Academy of Agriculture Sciences/Guangdong Provincial Key Laboratory of Crops Genetics and Improvement Guangdong/Guangdong Provincial Engineering & Technology Research Center for Conservation and Utilization of the Genuine Southern Medicinal Resources, Guangzhou, Guangdong, China

https://orcid.org/0000-0001-7670-5574

Yu Mei

Roles Formal analysis, Writing – original draft, Writing – review & editing

Jihua Wang

Roles Supervision, Writing – original draft, Writing – review & editing

* E-mail: wangjihua@gdaas.cn

Competing Interests

The authors have declared that no competing interests exist.

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Plant genomics
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Tomatoes
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Flow cytometry
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Fluorescent in situ hybridization
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Polyploidy
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Telomeres
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