Revolutionizing Molecular cloning: Introducing FastCloneAssist, a Streamlined Python tool for optimizing primer design in restriction & ligation-independent PCR cloning

Pradip Kumar Singh; Michael S. Donnenberg

doi:10.1371/journal.pone.0306950

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Open Access

Peer-reviewed

Research Article

Revolutionizing Molecular cloning: Introducing FastCloneAssist, a Streamlined Python tool for optimizing primer design in restriction & ligation-independent PCR cloning

Pradip Kumar Singh,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Resources, Software, Validation, Visualization, Writing – original draft, Writing – review & editing

Affiliation Virginia Commonwealth University, Richmond, Virginia, United States of America

https://orcid.org/0000-0003-0551-6877

⨯
Michael S. Donnenberg

Roles Funding acquisition, Investigation, Supervision, Writing – review & editing

* E-mail: michael.donnenberg@vcuhealth.org

Affiliation Virginia Commonwealth University, Richmond, Virginia, United States of America

https://orcid.org/0000-0002-8818-3184

⨯

Revolutionizing Molecular cloning: Introducing FastCloneAssist, a Streamlined Python tool for optimizing primer design in restriction & ligation-independent PCR cloning

Pradip Kumar Singh,
Michael S. Donnenberg

Published: March 13, 2025
https://doi.org/10.1371/journal.pone.0306950

About the Authors

Pradip Kumar Singh

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Resources, Software, Validation, Visualization, Writing – original draft, Writing – review & editing

Affiliation Virginia Commonwealth University, Richmond, Virginia, United States of America

https://orcid.org/0000-0003-0551-6877

Michael S. Donnenberg

Roles Funding acquisition, Investigation, Supervision, Writing – review & editing

* E-mail: michael.donnenberg@vcuhealth.org

Affiliation Virginia Commonwealth University, Richmond, Virginia, United States of America

https://orcid.org/0000-0002-8818-3184

Competing Interests

NO authors have competing interests.

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Cloning
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Polymerase chain reaction
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Melting
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Cloud computing
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Graphical user interfaces
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Molecular biology
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Nucleotide sequencing
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Vector cloning
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