About the Authors

Kamilla Kolding Bendixen

Roles Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Validation, Visualization, Writing – original draft, Writing – review & editing

Affiliation PentaBase A/S, Odense, Denmark

Sofie Forsberg-Pho

Roles Writing – original draft, Writing – review & editing

Affiliation PentaBase A/S, Odense, Denmark

Giulia Dazio

Roles Investigation, Resources, Validation, Writing – review & editing

Affiliation Institute of Pathology, Ente Ospedaliero Cantonale, Locarno, Switzerland

Emeli Elisabeth Hansen

Roles Data curation, Methodology

Affiliation PentaBase A/S, Odense, Denmark

Sarah Kronborg Eriksen

Roles Data curation, Methodology, Resources

Affiliation PentaBase A/S, Odense, Denmark

Samantha Epistolio

Roles Investigation, Resources, Validation, Writing – review & editing

Affiliation Institute of Pathology, Ente Ospedaliero Cantonale, Locarno, Switzerland

Elisabetta Merlo

Roles Investigation, Resources, Validation

Affiliation Institute of Pathology, Ente Ospedaliero Cantonale, Locarno, Switzerland

Renzo Boldorini

Roles Investigation, Resources, Validation

Affiliation Unit of Pathology, Department of Health Sciences, University of Eastern Piedmont, Novara, Italy

Tiziana Venesio

Roles Investigation, Resources, Validation

Affiliation Candiolo Cancer Institute, Fondazione del Piemonte per l’Oncologia, Candiolo, Italy

Alessandra Movilia

Roles Investigation, Resources, Validation

Affiliation Hospital of Legnano, SS Biologia Molecolare, UO Anatomia Patologica, Azienda Socio Sanitaria Territoriale Ovest Milanese, Ospedale di Legnano, Legnano, Italy

Cecilia Caprera

Roles Investigation, Resources, Validation

Affiliation Laboratory of Molecular Oncology and Predictive Medicine, Pathology Unit, Azienda Ospedaliera Santa Maria di Terni, Terni, Italy

Eva Christensen Arnspang

Roles Supervision

Affiliation Department of Green Technology, Faculty of Engineering, University of Southern Denmark, Odense, Denmark

Michael Børgesen

Roles Formal analysis

Affiliation PentaBase A/S, Odense, Denmark

Ulf Bech Christensen

Roles Conceptualization, Funding acquisition, Methodology

Affiliation PentaBase A/S, Odense, Denmark

Milo Frattini

Contributed equally to this work with: Milo Frattini, Rasmus Koefoed Petersen

Roles Project administration, Resources, Supervision, Visualization, Writing – review & editing

Affiliation Institute of Pathology, Ente Ospedaliero Cantonale, Locarno, Switzerland

Rasmus Koefoed Petersen

Contributed equally to this work with: Milo Frattini, Rasmus Koefoed Petersen

Roles Conceptualization, Formal analysis, Funding acquisition, Methodology, Project administration, Supervision, Visualization, Writing – original draft, Writing – review & editing

rkp@pentabase.com

Affiliation PentaBase A/S, Odense, Denmark

Competing Interests

I have read the journal’s policy and the authors of this manuscript have the following competing interests: K.K.B., E.E.H., S.K.E., M.B., U.B.C., and R.K.P. received salaries from Innovation Fund Denmark. The funding organization did not play a role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript and only provided financial support in the form of authors’ salaries and research materials. K.K.B., S.FP., E.E.H., S.K.E., M.B., U.B.C., and R.K.P. were employed at PentaBase A/S during this study. PentaBase A/S is the manufacturer and seller of MicroSight® MSI 1-step HRM Analysis, which is the assay of interest in this study. K.K.B., E.E.H., S.K.E., U.B.C., and R.K.P. are all founders of a patent application covering the novel technology which MicroSight® MSI 1-step HRM Analysis is based on [International Publication number: WO2020229510A1]. The remaining authors declare no conflict of interest. This does not alter our adherence to PLOS ONE policies on sharing data and materials.