Development and evaluation of TaqMan-based, one-step, real-time RT-PCR assays for pepper mild mottle virus detection for near source tracking and wastewater-based epidemiology validation

Daire Cantillon; Adam P. Roberts

doi:10.1371/journal.pone.0278784

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Open Access

Peer-reviewed

Research Article

Development and evaluation of TaqMan-based, one-step, real-time RT-PCR assays for pepper mild mottle virus detection for near source tracking and wastewater-based epidemiology validation

Daire Cantillon ,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Writing – original draft, Writing – review & editing

* E-mail: daire.cantillon@lstmed.ac.uk

Affiliation Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom

https://orcid.org/0000-0002-2180-373X

⨯
Adam P. Roberts

Roles Conceptualization, Funding acquisition, Methodology, Resources, Visualization, Writing – review & editing

Affiliation Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom

https://orcid.org/0000-0002-0760-3088

⨯

Development and evaluation of TaqMan-based, one-step, real-time RT-PCR assays for pepper mild mottle virus detection for near source tracking and wastewater-based epidemiology validation

Daire Cantillon,
Adam P. Roberts

Published: December 19, 2022
https://doi.org/10.1371/journal.pone.0278784

About the Authors

Daire Cantillon

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Writing – original draft, Writing – review & editing

* E-mail: daire.cantillon@lstmed.ac.uk

Affiliation Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom

https://orcid.org/0000-0002-2180-373X

Adam P. Roberts

Roles Conceptualization, Funding acquisition, Methodology, Resources, Visualization, Writing – review & editing

Affiliation Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom

https://orcid.org/0000-0002-0760-3088

Competing Interests

The authors have declared that no competing interests exist.

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Reverse transcriptase-polymerase chain reaction
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Viral pathogens
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RNA extraction
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SARS CoV 2
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Sewage
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RNA viruses
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Genomics
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