OverFlap PCR: A reliable approach for generating plasmid DNA libraries containing random sequences without a template bias

Open Access

Peer-reviewed

Research Article

About the Authors

Artis Linars

Roles Investigation

Affiliation Latvian Biomedical Research and Study Centre, Riga, Latvia

Ivars Silamikelis

Roles Investigation, Software, Writing – review & editing

Affiliation Latvian Biomedical Research and Study Centre, Riga, Latvia

Dita Gudra

Roles Investigation, Resources, Writing – review & editing

Affiliation Latvian Biomedical Research and Study Centre, Riga, Latvia

Ance Roga

Roles Conceptualization, Investigation, Writing – review & editing

Affiliation Latvian Biomedical Research and Study Centre, Riga, Latvia

Davids Fridmanis

Roles Conceptualization, Funding acquisition, Investigation, Supervision, Writing – original draft, Writing – review & editing

Affiliation Latvian Biomedical Research and Study Centre, Riga, Latvia

The authors have declared that no competing interests exist.

Polymerase chain reaction
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Plasmid construction
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Nucleotide sequencing
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Peptide libraries
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Mutagenesis
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Oligonucleotides
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Signal amplification
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Saccharomyces cerevisiae
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