Disentangling primer interactions improves SARS-CoV-2 genome sequencing by multiplex tiling PCR

Open Access

Peer-reviewed

Research Article

About the Authors

Kentaro Itokawa

Roles Conceptualization, Investigation, Visualization, Writing – original draft

Affiliation Pathogen Genomics Center, National Institute of Infectious Diseases, Tokyo, Japan

Tsuyoshi Sekizuka

Roles Conceptualization, Validation, Writing – review & editing

Affiliation Pathogen Genomics Center, National Institute of Infectious Diseases, Tokyo, Japan

Masanori Hashino

Roles Investigation

Affiliation Pathogen Genomics Center, National Institute of Infectious Diseases, Tokyo, Japan

Rina Tanaka

Roles Investigation

Affiliation Pathogen Genomics Center, National Institute of Infectious Diseases, Tokyo, Japan

Makoto Kuroda

Roles Conceptualization, Funding acquisition, Writing – review & editing

Affiliation Pathogen Genomics Center, National Institute of Infectious Diseases, Tokyo, Japan

The authors have declared that no competing interests exist.

Polymerase chain reaction
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SARS CoV 2
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Viral load
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COVID 19
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Genomics
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Genome sequencing
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Temperature gradients
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Viral genome
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