First echinoderm alpha-amylase from a tropical sea cucumber (Holothuria leucospilota): Molecular cloning, tissue distribution, cellular localization and functional production in a heterogenous E.coli system with codon optimization

• Xiaofen Wu ,

  Contributed equally to this work with: Xiaofen Wu, Yao Ruan

  Roles Data curation, Formal analysis, Investigation, Methodology, Visualization, Writing – original draft

  Affiliations CAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB), Guangdong Provincial Key Laboratory of Applied Marine Biology (LAMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, PR China, University of Chinese Academy of Sciences, Beijing, PR China

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• Yao Ruan ,

  Contributed equally to this work with: Xiaofen Wu, Yao Ruan

  Roles Investigation, Methodology, Validation

  Affiliations CAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB), Guangdong Provincial Key Laboratory of Applied Marine Biology (LAMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, PR China, University of Chinese Academy of Sciences, Beijing, PR China

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• Ting Chen,

  Roles Conceptualization, Data curation, Methodology, Supervision, Validation, Writing – review & editing

  Affiliations CAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB), Guangdong Provincial Key Laboratory of Applied Marine Biology (LAMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, PR China, Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou, PR China, Institution of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences, ISEE, CAS, Guangzhou, PR China

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• Zonghe Yu,

  Roles Funding acquisition, Resources

  Affiliation College of Marine Sciences, South China Agricultural University, Guangzhou, PR China

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• Da Huo,

  Roles Conceptualization, Formal analysis, Methodology

  Affiliations CAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB), Guangdong Provincial Key Laboratory of Applied Marine Biology (LAMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, PR China, Institution of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences, ISEE, CAS, Guangzhou, PR China

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• Xiaomin Li,

  Roles Methodology, Software, Validation

  Affiliations CAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB), Guangdong Provincial Key Laboratory of Applied Marine Biology (LAMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, PR China, University of Chinese Academy of Sciences, Beijing, PR China

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• Feifei Wu,

  Roles Formal analysis, Resources, Validation, Writing – review & editing

  Affiliations CAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB), Guangdong Provincial Key Laboratory of Applied Marine Biology (LAMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, PR China, University of Chinese Academy of Sciences, Beijing, PR China

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• Xiao Jiang,

  Roles Data curation, Funding acquisition, Resources

  Affiliations CAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB), Guangdong Provincial Key Laboratory of Applied Marine Biology (LAMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, PR China, Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou, PR China, Institution of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences, ISEE, CAS, Guangzhou, PR China

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• Chunhua Ren

  Roles Conceptualization, Data curation, Funding acquisition, Project administration, Writing – review & editing

  * E-mail: rosemary166@sina.com

  Affiliations CAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB), Guangdong Provincial Key Laboratory of Applied Marine Biology (LAMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, PR China, Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou, PR China, Institution of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences, ISEE, CAS, Guangzhou, PR China

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