About the Authors

Pedro J. Alcolea

Contributed equally to this work with: Pedro J. Alcolea, Ana Alonso

Roles Conceptualization, Formal analysis, Investigation, Methodology, Resources, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

pjalcolea@cib.csic.es

Affiliation Laboratory of Molecular Parasitology, Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Madrid, Spain

Ana Alonso

Contributed equally to this work with: Pedro J. Alcolea, Ana Alonso

Roles Data curation, Investigation, Methodology, Validation, Visualization, Writing – review & editing

Affiliation Laboratory of Molecular Parasitology, Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Madrid, Spain

Adriana Esteban

Roles Methodology, Resources, Visualization

Affiliation Laboratory of Parasitology, Department of Pathology, Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, Spain

Paz Peris

Roles Methodology, Resources, Visualization

Affiliation Laboratory of Parasitology, Department of Pathology, Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, Spain

Alberto Cortés

Roles Methodology, Resources, Visualization

Affiliation Laboratory of Parasitology, Department of Pathology, Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, Spain

Juan A. Castillo

Roles Conceptualization, Methodology, Resources, Supervision

Affiliation Laboratory of Parasitology, Department of Pathology, Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, Spain

Vicente Larraga

Roles Conceptualization, Data curation, Funding acquisition, Resources, Supervision, Writing – review & editing

Affiliation Laboratory of Molecular Parasitology, Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Madrid, Spain

Competing Interests

The authors declare that they do not have conflict of interests. Funding by CZ Veterinaria - Biofabri was performed for the sole purpose of acquiring inventoriable and consumable material for experimentation. PJA, AA, JAC, and VL are official public servants of the Spanish Ministry of Science, Innovation and Universities, and all other authors did not receive any funds for employment by CZ Veterinaria - Biofabri for experimentation described in the manuscript. The pPAL vector and the pPAL-LACK vaccine used in this study were patented as cited in the manuscript body, but the pPAL-IL12p35 and pPALIL12p40 plasmid constructs, which are the specific research subject of this manuscript, are not protected by patent. All steps of the procedure have been exhaustively detailed, and all data are available without restriction. Therefore, we declare that this does not alter our adherence to PLOS ONE policies on sharing data and materials.