In vivo assembly and large-scale purification of a GPCR - Gα fusion with Gβγ, and characterization of the active complex

Open Access

Peer-reviewed

Research Article

About the Authors

Abhinav Kumar

Roles Conceptualization, Investigation, Methodology, Writing – original draft

Affiliation Department of Biochemistry, University of Zürich, Zürich, Switzerland

Andreas Plückthun

Roles Conceptualization, Funding acquisition, Project administration, Supervision, Writing – review & editing

Affiliation Department of Biochemistry, University of Zürich, Zürich, Switzerland

The authors have declared that no competing interests exist.

Detergents
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Proteases
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G protein coupled receptors
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Protein complexes
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Membrane fusion
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Yellow fluorescent protein
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Elution
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Insects
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