Direct and Inverted Repeat stimulated excision (DIRex): Simple, single-step, and scar-free mutagenesis of bacterial genes

Joakim Näsvall

doi:10.1371/journal.pone.0184126

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Open Access

Peer-reviewed

Research Article

Direct and Inverted Repeat stimulated excision (DIRex): Simple, single-step, and scar-free mutagenesis of bacterial genes

Joakim Näsvall

Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Validation, Visualization, Writing – original draft, Writing – review & editing

* E-mail: joakim.nasvall@imbim.uu.se

Affiliation Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden

http://orcid.org/0000-0002-6831-3105

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Direct and Inverted Repeat stimulated excision (DIRex): Simple, single-step, and scar-free mutagenesis of bacterial genes

Joakim Näsvall

Published: August 30, 2017
https://doi.org/10.1371/journal.pone.0184126
See the preprint

About the Authors

Joakim Näsvall

Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Validation, Visualization, Writing – original draft, Writing – review & editing

* E-mail: joakim.nasvall@imbim.uu.se

Affiliation Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden

http://orcid.org/0000-0002-6831-3105

Competing Interests

The authors have declared that no competing interests exist.

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Point mutation
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Polymerase chain reaction
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DNA recombination
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Cloning
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Homologous recombination
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Transformation associated recombination
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Salmonella enterica
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Frameshift mutation
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