A novel, multiplexed, probe-based quantitative PCR assay for the soybean root- and stem-rot pathogen, Phytophthora sojae, utilizes its transposable element

James S. Haudenshield; Jeong Y. Song; Glen L. Hartman

doi:10.1371/journal.pone.0176567

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Peer-reviewed

Research Article

A novel, multiplexed, probe-based quantitative PCR assay for the soybean root- and stem-rot pathogen, Phytophthora sojae, utilizes its transposable element

James S. Haudenshield,

Affiliation United States Department of Agriculture-Agricultural Research Service, and Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States of America
⨯
Jeong Y. Song,

Current address: Department of Applied Biology, Chungnam National University, Daejeon, Korea

Affiliation Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States of America
⨯
Glen L. Hartman

* E-mail: ghartman@illinois.edu

Affiliation United States Department of Agriculture-Agricultural Research Service, and Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States of America
⨯

A novel, multiplexed, probe-based quantitative PCR assay for the soybean root- and stem-rot pathogen, Phytophthora sojae, utilizes its transposable element

James S. Haudenshield,
Jeong Y. Song,
Glen L. Hartman

Published: April 25, 2017
https://doi.org/10.1371/journal.pone.0176567

About the Authors

James S. Haudenshield

Affiliation United States Department of Agriculture-Agricultural Research Service, and Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States of America

Jeong Y. Song

Current address: Department of Applied Biology, Chungnam National University, Daejeon, Korea

Affiliation Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States of America

Glen L. Hartman

* E-mail: ghartman@illinois.edu

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceptualization: JSH JYS GLH. Data curation: JSH GLH. Formal analysis: JSH. Funding acquisition: JYS GLH. Investigation: JSH JYS GLH. Methodology: JSH JYS GLH. Project administration: GLH. Resources: JSH GLH. Supervision: GLH. Validation: JSH. Visualization: JSH. Writing – original draft: JSH. Writing – review & editing: JSH GLH.

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Phytophthora
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Soybeans
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Signal amplification
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DNA isolation
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Polymerase chain reaction
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Oomycetes
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DNA extraction
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Fungal pathogens
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