S1-Type Endonuclease 2 in Dedifferentiating Arabidopsis Protoplasts: Translocation to the Nucleus in Senescing Protoplasts Is Associated with De-Glycosylation

Yemima Givaty-Rapp; Narendra Singh Yadav; Asif Khan; Gideon Grafi

doi:10.1371/journal.pone.0170067

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Open Access

Peer-reviewed

Research Article

S1-Type Endonuclease 2 in Dedifferentiating Arabidopsis Protoplasts: Translocation to the Nucleus in Senescing Protoplasts Is Associated with De-Glycosylation

Yemima Givaty-Rapp,

Affiliation French Associates Institute of Agriculture and Biotechnology of Drylands, The Institutes for Desert Research, Ben-Gurion University of the Negev, Midreshet Ben-Gurion, Israel
⨯
Narendra Singh Yadav,

Affiliation French Associates Institute of Agriculture and Biotechnology of Drylands, The Institutes for Desert Research, Ben-Gurion University of the Negev, Midreshet Ben-Gurion, Israel
⨯
Asif Khan,

Affiliation French Associates Institute of Agriculture and Biotechnology of Drylands, The Institutes for Desert Research, Ben-Gurion University of the Negev, Midreshet Ben-Gurion, Israel
⨯
Gideon Grafi

* E-mail: ggrafi@bgu.ac.il

Affiliation French Associates Institute of Agriculture and Biotechnology of Drylands, The Institutes for Desert Research, Ben-Gurion University of the Negev, Midreshet Ben-Gurion, Israel
⨯

S1-Type Endonuclease 2 in Dedifferentiating Arabidopsis Protoplasts: Translocation to the Nucleus in Senescing Protoplasts Is Associated with De-Glycosylation

Yemima Givaty-Rapp,
Narendra Singh Yadav,
Asif Khan,
Gideon Grafi

Published: January 9, 2017
https://doi.org/10.1371/journal.pone.0170067

About the Authors

Yemima Givaty-Rapp

Affiliation French Associates Institute of Agriculture and Biotechnology of Drylands, The Institutes for Desert Research, Ben-Gurion University of the Negev, Midreshet Ben-Gurion, Israel

Narendra Singh Yadav

Affiliation French Associates Institute of Agriculture and Biotechnology of Drylands, The Institutes for Desert Research, Ben-Gurion University of the Negev, Midreshet Ben-Gurion, Israel

Asif Khan

Affiliation French Associates Institute of Agriculture and Biotechnology of Drylands, The Institutes for Desert Research, Ben-Gurion University of the Negev, Midreshet Ben-Gurion, Israel

Gideon Grafi

* E-mail: ggrafi@bgu.ac.il

Affiliation French Associates Institute of Agriculture and Biotechnology of Drylands, The Institutes for Desert Research, Ben-Gurion University of the Negev, Midreshet Ben-Gurion, Israel

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceptualization: GG YG-R. Formal analysis: YG-R. Funding acquisition: GG. Investigation: YG-R NSY AK. Methodology: YG-R AK. Supervision: GG. Validation: YG-R NSY AK. Writing – original draft: GG. Writing – review & editing: GG YG-R NSY AK.

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Nucleases
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Chromatin
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Arabidopsis thaliana
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Protein extraction
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Leaves
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DNA denaturation
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Cytoplasm
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Polymerase chain reaction
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