Cross-Neutralising Nanobodies Bind to a Conserved Pocket in the Hemagglutinin Stem Region Identified Using Yeast Display and Deep Mutational Scanning

Tiziano Gaiotto; Simon E. Hufton

doi:10.1371/journal.pone.0164296

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Open Access

Peer-reviewed

Research Article

Cross-Neutralising Nanobodies Bind to a Conserved Pocket in the Hemagglutinin Stem Region Identified Using Yeast Display and Deep Mutational Scanning

Tiziano Gaiotto,

Affiliation Biotherapeutics Group, National Institute for Biological Standards and Control, a centre of the Medicines and Healthcare Products Regulatory Agency, Blanche Lane, South Mimms, Potters Bar, Herts, EN6 3QG, United Kingdom
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Simon E. Hufton

* E-mail: simon.hufton@nibsc.org

Affiliation Biotherapeutics Group, National Institute for Biological Standards and Control, a centre of the Medicines and Healthcare Products Regulatory Agency, Blanche Lane, South Mimms, Potters Bar, Herts, EN6 3QG, United Kingdom
⨯

Cross-Neutralising Nanobodies Bind to a Conserved Pocket in the Hemagglutinin Stem Region Identified Using Yeast Display and Deep Mutational Scanning

Tiziano Gaiotto,
Simon E. Hufton

Published: October 14, 2016
https://doi.org/10.1371/journal.pone.0164296

About the Authors

Tiziano Gaiotto

Affiliation Biotherapeutics Group, National Institute for Biological Standards and Control, a centre of the Medicines and Healthcare Products Regulatory Agency, Blanche Lane, South Mimms, Potters Bar, Herts, EN6 3QG, United Kingdom

Simon E. Hufton

* E-mail: simon.hufton@nibsc.org

Competing Interests

An international patent application (PCT/GB2012/052164) covering the monoclonal antibodies described in this study has been filed. This does not alter adherence to all of the PLOS ONE policies on sharing data and information.

Author Contributions

Conceptualization: TG SEH. Investigation: TG SEH. Methodology: TG SEH. Project administration: SEH. Supervision: SEH. Visualization: TG. Writing – original draft: TG SEH. Writing – review & editing: TG SEH.

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Antibodies
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Yeast
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H1N1
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Point mutation
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Flow cytometry
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Binding analysis
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Mutation detection
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Epitope mapping
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