Practical Recommendations for the Use of the GeneSwitch Gal4 System to Knock-Down Genes in Drosophila melanogaster

Filippo Scialo; Ashwin Sriram; Rhoda Stefanatos; Alberto Sanz

doi:10.1371/journal.pone.0161817

Loading metrics

Open Access

Peer-reviewed

Research Article

Practical Recommendations for the Use of the GeneSwitch Gal4 System to Knock-Down Genes in Drosophila melanogaster

Filippo Scialo,

Affiliation Institute for Cell and Molecular Biosciences, Campus for Ageing and Vitality, University of Newcastle, Newcastle-upon-Tyne, NE4 5PL, United Kingdom
⨯
Ashwin Sriram,

Affiliation Institute for Cell and Molecular Biosciences, Campus for Ageing and Vitality, University of Newcastle, Newcastle-upon-Tyne, NE4 5PL, United Kingdom
⨯
Rhoda Stefanatos,

Affiliation Institute for Cell and Molecular Biosciences, Campus for Ageing and Vitality, University of Newcastle, Newcastle-upon-Tyne, NE4 5PL, United Kingdom
⨯
Alberto Sanz

* E-mail: Alberto.Sanz@ncl.ac.uk

Affiliation Institute for Cell and Molecular Biosciences, Campus for Ageing and Vitality, University of Newcastle, Newcastle-upon-Tyne, NE4 5PL, United Kingdom
⨯

Practical Recommendations for the Use of the GeneSwitch Gal4 System to Knock-Down Genes in Drosophila melanogaster

Filippo Scialo,
Ashwin Sriram,
Rhoda Stefanatos,
Alberto Sanz

Published: August 29, 2016
https://doi.org/10.1371/journal.pone.0161817

About the Authors

Filippo Scialo

Affiliation Institute for Cell and Molecular Biosciences, Campus for Ageing and Vitality, University of Newcastle, Newcastle-upon-Tyne, NE4 5PL, United Kingdom

Ashwin Sriram

Affiliation Institute for Cell and Molecular Biosciences, Campus for Ageing and Vitality, University of Newcastle, Newcastle-upon-Tyne, NE4 5PL, United Kingdom

Rhoda Stefanatos

Affiliation Institute for Cell and Molecular Biosciences, Campus for Ageing and Vitality, University of Newcastle, Newcastle-upon-Tyne, NE4 5PL, United Kingdom

Alberto Sanz

* E-mail: Alberto.Sanz@ncl.ac.uk

Affiliation Institute for Cell and Molecular Biosciences, Campus for Ageing and Vitality, University of Newcastle, Newcastle-upon-Tyne, NE4 5PL, United Kingdom

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceptualization: A. Sanz. Formal analysis: FS A. Sriram RS A. Sanz. Funding acquisition: A. Sanz. Investigation: FS A. Sriram RS A. Sanz. Methodology: A. Sanz. Project administration: A. Sanz. Supervision: A. Sanz. Validation: FS A. Sriram. Visualization: FS. Writing – original draft: A. Sanz. Writing – review & editing: FS RS A. Sanz.

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

RNA interference
Is the Subject Area "RNA interference" applicable to this article?

Thanks for your feedback.
Gene expression
Is the Subject Area "Gene expression" applicable to this article?

Thanks for your feedback.
Drosophila melanogaster
Is the Subject Area "Drosophila melanogaster" applicable to this article?

Thanks for your feedback.
Protein expression
Is the Subject Area "Protein expression" applicable to this article?

Thanks for your feedback.
Progesterone
Is the Subject Area "Progesterone" applicable to this article?

Thanks for your feedback.
Tubulins
Is the Subject Area "Tubulins" applicable to this article?

Thanks for your feedback.
Hyperexpression techniques
Is the Subject Area "Hyperexpression techniques" applicable to this article?

Thanks for your feedback.
Transcription factors
Is the Subject Area "Transcription factors" applicable to this article?

Thanks for your feedback.