Microsatellite Length Scoring by Single Molecule Real Time Sequencing – Effects of Sequence Structure and PCR Regime

Mikkel Meyn Liljegren; Eric Jacques de Muinck; Pål Trosvik

doi:10.1371/journal.pone.0159232

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Open Access

Peer-reviewed

Research Article

Microsatellite Length Scoring by Single Molecule Real Time Sequencing – Effects of Sequence Structure and PCR Regime

Mikkel Meyn Liljegren,

Affiliation Centre for Ecological and Evolutionary Synthesis, Dept. of Biosciences, University of Oslo, Oslo, Norway
⨯
Eric Jacques de Muinck,

Affiliation Centre for Ecological and Evolutionary Synthesis, Dept. of Biosciences, University of Oslo, Oslo, Norway
⨯
Pål Trosvik

* E-mail: pal.trosvik@ibv.uio.no

Affiliation Centre for Ecological and Evolutionary Synthesis, Dept. of Biosciences, University of Oslo, Oslo, Norway
⨯

Microsatellite Length Scoring by Single Molecule Real Time Sequencing – Effects of Sequence Structure and PCR Regime

Mikkel Meyn Liljegren,
Eric Jacques de Muinck,
Pål Trosvik

Published: July 14, 2016
https://doi.org/10.1371/journal.pone.0159232

About the Authors

Mikkel Meyn Liljegren

Affiliation Centre for Ecological and Evolutionary Synthesis, Dept. of Biosciences, University of Oslo, Oslo, Norway

Eric Jacques de Muinck

Affiliation Centre for Ecological and Evolutionary Synthesis, Dept. of Biosciences, University of Oslo, Oslo, Norway

Pål Trosvik

* E-mail: pal.trosvik@ibv.uio.no

Affiliation Centre for Ecological and Evolutionary Synthesis, Dept. of Biosciences, University of Oslo, Oslo, Norway

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: MML EJM PT. Performed the experiments: MML EJM PT. Analyzed the data: MML EJM PT. Contributed reagents/materials/analysis tools: MML EJM PT. Wrote the paper: MML EJM PT.

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Polymerase chain reaction
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Insertion mutation
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Deletion mutation
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Nucleotide sequencing
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Plasmid construction
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Agarose gel electrophoresis
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Capillary electrophoresis
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DNA sequencing
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