Characterization of an Indole-3-Acetamide Hydrolase from Alcaligenes faecalis subsp. parafaecalis and Its Application in Efficient Preparation of Both Enantiomers of Chiral Building Block 2,3-Dihydro-1,4-Benzodioxin-2-Carboxylic Acid

Pradeep Mishra; Suneet Kaur; Amar Nath Sharma; Ravinder S. Jolly

doi:10.1371/journal.pone.0159009

Loading metrics

Open Access

Peer-reviewed

Research Article

Characterization of an Indole-3-Acetamide Hydrolase from Alcaligenes faecalis subsp. parafaecalis and Its Application in Efficient Preparation of Both Enantiomers of Chiral Building Block 2,3-Dihydro-1,4-Benzodioxin-2-Carboxylic Acid

Pradeep Mishra,

Affiliation Department of Bioorganic Chemistry, CSIR-Institute of Microbial Technology, Sector 39, Chandigarh, India
⨯
Suneet Kaur,

Current address: Department of Medicine, Duke University Medical Center, Durham, North Carolina, United States of America

Affiliation Department of Bioorganic Chemistry, CSIR-Institute of Microbial Technology, Sector 39, Chandigarh, India
⨯
Amar Nath Sharma,

Affiliation Department of Bioorganic Chemistry, CSIR-Institute of Microbial Technology, Sector 39, Chandigarh, India
⨯
Ravinder S. Jolly

* E-mail: jolly@imtech.res.in

Affiliation Department of Bioorganic Chemistry, CSIR-Institute of Microbial Technology, Sector 39, Chandigarh, India
⨯

Characterization of an Indole-3-Acetamide Hydrolase from Alcaligenes faecalis subsp. parafaecalis and Its Application in Efficient Preparation of Both Enantiomers of Chiral Building Block 2,3-Dihydro-1,4-Benzodioxin-2-Carboxylic Acid

Pradeep Mishra,
Suneet Kaur,
Amar Nath Sharma,
Ravinder S. Jolly

Published: July 8, 2016
https://doi.org/10.1371/journal.pone.0159009

About the Authors

Pradeep Mishra

Affiliation Department of Bioorganic Chemistry, CSIR-Institute of Microbial Technology, Sector 39, Chandigarh, India

Suneet Kaur

Current address: Department of Medicine, Duke University Medical Center, Durham, North Carolina, United States of America

Affiliation Department of Bioorganic Chemistry, CSIR-Institute of Microbial Technology, Sector 39, Chandigarh, India

Amar Nath Sharma

Affiliation Department of Bioorganic Chemistry, CSIR-Institute of Microbial Technology, Sector 39, Chandigarh, India

Ravinder S. Jolly

* E-mail: jolly@imtech.res.in

Affiliation Department of Bioorganic Chemistry, CSIR-Institute of Microbial Technology, Sector 39, Chandigarh, India

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: PM RSJ. Performed the experiments: PM SK ANS. Analyzed the data: PM SK ANS RSJ. Contributed reagents/materials/analysis tools: PM SK ANS. Wrote the paper: PM RSJ.

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

Amides
Is the Subject Area "Amides" applicable to this article?

Thanks for your feedback.
Biocatalysis
Is the Subject Area "Biocatalysis" applicable to this article?

Thanks for your feedback.
Hydrolases
Is the Subject Area "Hydrolases" applicable to this article?

Thanks for your feedback.
Enantiomers
Is the Subject Area "Enantiomers" applicable to this article?

Thanks for your feedback.
Flow rate
Is the Subject Area "Flow rate" applicable to this article?

Thanks for your feedback.
Gel electrophoresis
Is the Subject Area "Gel electrophoresis" applicable to this article?

Thanks for your feedback.
Size-exclusion chromatography
Is the Subject Area "Size-exclusion chromatography" applicable to this article?

Thanks for your feedback.
Sulfates
Is the Subject Area "Sulfates" applicable to this article?

Thanks for your feedback.