Mutation in the Pro-Peptide Region of a Cysteine Protease Leads to Altered Activity and Specificity—A Structural and Biochemical Approach

Sruti Dutta; Debi Choudhury; Sumana Roy; Jiban Kanti Dattagupta; Sampa Biswas

doi:10.1371/journal.pone.0158024

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Open Access

Peer-reviewed

Research Article

Mutation in the Pro-Peptide Region of a Cysteine Protease Leads to Altered Activity and Specificity—A Structural and Biochemical Approach

Sruti Dutta,

Affiliation Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata, 700 064, India
⨯
Debi Choudhury,

Affiliation Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata, 700 064, India
⨯
Sumana Roy,

Affiliation Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata, 700 064, India
⨯
Jiban Kanti Dattagupta,

Affiliation Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata, 700 064, India
⨯
Sampa Biswas

* E-mail: sampa.biswas@saha.ac.in

Affiliation Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata, 700 064, India
⨯

Mutation in the Pro-Peptide Region of a Cysteine Protease Leads to Altered Activity and Specificity—A Structural and Biochemical Approach

Sruti Dutta,
Debi Choudhury,
Sumana Roy,
Jiban Kanti Dattagupta,
Sampa Biswas

Published: June 28, 2016
https://doi.org/10.1371/journal.pone.0158024

About the Authors

Sruti Dutta

Affiliation Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata, 700 064, India

Debi Choudhury

Affiliation Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata, 700 064, India

Sumana Roy

Affiliation Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata, 700 064, India

Jiban Kanti Dattagupta

Affiliation Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata, 700 064, India

Sampa Biswas

* E-mail: sampa.biswas@saha.ac.in

Affiliation Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata, 700 064, India

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: SB. Performed the experiments: SB SD SR DC. Analyzed the data: SB DC. Contributed reagents/materials/analysis tools: SB JKD. Wrote the paper: SB JKD.

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Proteases
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Cysteine proteases
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Zymogens
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Crystal structure
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Enzyme structure
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Point mutation
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Enzymes
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Enzyme kinetics
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