Dual Targeting of Akt and mTORC1 Impairs Repair of DNA Double-Strand Breaks and Increases Radiation Sensitivity of Human Tumor Cells

Marina Holler; Astrid Grottke; Katharina Mueck; Julia Manes; Manfred Jücker; H. Peter Rodemann; Mahmoud Toulany

doi:10.1371/journal.pone.0154745

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Open Access

Peer-reviewed

Research Article

Dual Targeting of Akt and mTORC1 Impairs Repair of DNA Double-Strand Breaks and Increases Radiation Sensitivity of Human Tumor Cells

Marina Holler,

Affiliation Division of Radiobiology and Molecular Environmental Research, Department of Radiation Oncology, Eberhard Karls University Tuebingen, Roentgenweg 11, 72076, Tuebingen, Germany
⨯
Astrid Grottke,

Affiliation Institute of Biochemistry and Signal Transduction, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246, Hamburg, Germany
⨯
Katharina Mueck,

Affiliation Division of Radiobiology and Molecular Environmental Research, Department of Radiation Oncology, Eberhard Karls University Tuebingen, Roentgenweg 11, 72076, Tuebingen, Germany
⨯
Julia Manes,

Affiliation Division of Radiobiology and Molecular Environmental Research, Department of Radiation Oncology, Eberhard Karls University Tuebingen, Roentgenweg 11, 72076, Tuebingen, Germany
⨯
Manfred Jücker,

Affiliation Institute of Biochemistry and Signal Transduction, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246, Hamburg, Germany
⨯
H. Peter Rodemann ,

Contributed equally to this work with: H. Peter Rodemann, Mahmoud Toulany

Affiliation Division of Radiobiology and Molecular Environmental Research, Department of Radiation Oncology, Eberhard Karls University Tuebingen, Roentgenweg 11, 72076, Tuebingen, Germany
⨯
Mahmoud Toulany

Contributed equally to this work with: H. Peter Rodemann, Mahmoud Toulany

* E-mail: mahmoud.toulany@uni-tuebingen.de

Affiliation Division of Radiobiology and Molecular Environmental Research, Department of Radiation Oncology, Eberhard Karls University Tuebingen, Roentgenweg 11, 72076, Tuebingen, Germany
⨯

Dual Targeting of Akt and mTORC1 Impairs Repair of DNA Double-Strand Breaks and Increases Radiation Sensitivity of Human Tumor Cells

Marina Holler,
Astrid Grottke,
Katharina Mueck,
Julia Manes,
Manfred Jücker,
H. Peter Rodemann,
Mahmoud Toulany

Published: May 3, 2016
https://doi.org/10.1371/journal.pone.0154745

About the Authors

Marina Holler

Affiliation Division of Radiobiology and Molecular Environmental Research, Department of Radiation Oncology, Eberhard Karls University Tuebingen, Roentgenweg 11, 72076, Tuebingen, Germany

Astrid Grottke

Affiliation Institute of Biochemistry and Signal Transduction, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246, Hamburg, Germany

Katharina Mueck

Affiliation Division of Radiobiology and Molecular Environmental Research, Department of Radiation Oncology, Eberhard Karls University Tuebingen, Roentgenweg 11, 72076, Tuebingen, Germany

Julia Manes

Affiliation Division of Radiobiology and Molecular Environmental Research, Department of Radiation Oncology, Eberhard Karls University Tuebingen, Roentgenweg 11, 72076, Tuebingen, Germany

Manfred Jücker

Affiliation Institute of Biochemistry and Signal Transduction, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246, Hamburg, Germany

H. Peter Rodemann

Contributed equally to this work with: H. Peter Rodemann, Mahmoud Toulany

Affiliation Division of Radiobiology and Molecular Environmental Research, Department of Radiation Oncology, Eberhard Karls University Tuebingen, Roentgenweg 11, 72076, Tuebingen, Germany

Mahmoud Toulany

Contributed equally to this work with: H. Peter Rodemann, Mahmoud Toulany

* E-mail: mahmoud.toulany@uni-tuebingen.de

Affiliation Division of Radiobiology and Molecular Environmental Research, Department of Radiation Oncology, Eberhard Karls University Tuebingen, Roentgenweg 11, 72076, Tuebingen, Germany

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: MT HPR MH. Performed the experiments: MH KM JM. Analyzed the data: MT HPR MH MJ. Contributed reagents/materials/analysis tools: MJ AG. Wrote the paper: MT HPR MH MJ.

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DNA repair
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Non-small cell lung cancer
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Transfection
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Breast cancer
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Non-homologous end joining
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Phosphorylation
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Radiation therapy
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