Two New Native β-Glucosidases from Clavispora NRRL Y-50464 Confer Its Dual Function as Cellobiose Fermenting Ethanologenic Yeast

Xu Wang; Z. Lewis Liu; Scott A. Weber; Xiaoping Zhang

doi:10.1371/journal.pone.0151293

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Open Access

Peer-reviewed

Research Article

Two New Native β-Glucosidases from Clavispora NRRL Y-50464 Confer Its Dual Function as Cellobiose Fermenting Ethanologenic Yeast

Xu Wang,

Affiliations Sichuan Agricultural University, Chengdu, China, Bioenergy Research Unit, National Center for Agricultural Utilization Research, USDA-ARS, Peoria, Illinois, 61604, United States of America
⨯
Z. Lewis Liu ,

* E-mail: ZLewis.Liu@ars.usda.gov

Affiliation Bioenergy Research Unit, National Center for Agricultural Utilization Research, USDA-ARS, Peoria, Illinois, 61604, United States of America
⨯
Scott A. Weber,

Affiliation Bioenergy Research Unit, National Center for Agricultural Utilization Research, USDA-ARS, Peoria, Illinois, 61604, United States of America
⨯
Xiaoping Zhang

Affiliation Sichuan Agricultural University, Chengdu, China
⨯

Two New Native β-Glucosidases from Clavispora NRRL Y-50464 Confer Its Dual Function as Cellobiose Fermenting Ethanologenic Yeast

Xu Wang,
Z. Lewis Liu,
Scott A. Weber,
Xiaoping Zhang

Published: March 24, 2016
https://doi.org/10.1371/journal.pone.0151293

About the Authors

Xu Wang

Affiliations Sichuan Agricultural University, Chengdu, China, Bioenergy Research Unit, National Center for Agricultural Utilization Research, USDA-ARS, Peoria, Illinois, 61604, United States of America

Z. Lewis Liu

* E-mail: ZLewis.Liu@ars.usda.gov

Affiliation Bioenergy Research Unit, National Center for Agricultural Utilization Research, USDA-ARS, Peoria, Illinois, 61604, United States of America

Scott A. Weber

Affiliation Bioenergy Research Unit, National Center for Agricultural Utilization Research, USDA-ARS, Peoria, Illinois, 61604, United States of America

Xiaoping Zhang

Affiliation Sichuan Agricultural University, Chengdu, China

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: XW ZLL XZ. Performed the experiments: XW SAW. Analyzed the data: XW ZLL. Contributed reagents/materials/analysis tools: XW ZLL. Wrote the paper: WX ZLL XZ.

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Ethanol
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Glucose
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Gene cloning
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Yeast
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Gene expression
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Enzyme assays
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Fermentation
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Enzyme inhibitors
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