Purification and Characterization of Glutaminase Free Asparaginase from Enterobacter cloacae: In-Vitro Evaluation of Cytotoxic Potential against Human Myeloid Leukemia HL-60 Cells

Islam Husain; Anjana Sharma; Suresh Kumar; Fayaz Malik

doi:10.1371/journal.pone.0148877

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Open Access

Peer-reviewed

Research Article

Purification and Characterization of Glutaminase Free Asparaginase from Enterobacter cloacae: In-Vitro Evaluation of Cytotoxic Potential against Human Myeloid Leukemia HL-60 Cells

Islam Husain,

Affiliation Bacteriology Laboratory, Department of P. G. Studies and Research in Biological Science, Rani Durgavati University, Jabalpur, Madhya Pradesh, India
⨯
Anjana Sharma ,

* E-mail: anjoo1999@gmail.com

Affiliation Bacteriology Laboratory, Department of P. G. Studies and Research in Biological Science, Rani Durgavati University, Jabalpur, Madhya Pradesh, India
⨯
Suresh Kumar,

Affiliation Cancer Pharmacology Division, Indian Institute of Integrative Medicine, Jammu, India
⨯
Fayaz Malik

Affiliation Cancer Pharmacology Division, Indian Institute of Integrative Medicine, Jammu, India
⨯

Purification and Characterization of Glutaminase Free Asparaginase from Enterobacter cloacae: In-Vitro Evaluation of Cytotoxic Potential against Human Myeloid Leukemia HL-60 Cells

Islam Husain,
Anjana Sharma,
Suresh Kumar,
Fayaz Malik

Published: February 18, 2016
https://doi.org/10.1371/journal.pone.0148877

About the Authors

Islam Husain

Affiliation Bacteriology Laboratory, Department of P. G. Studies and Research in Biological Science, Rani Durgavati University, Jabalpur, Madhya Pradesh, India

Anjana Sharma

* E-mail: anjoo1999@gmail.com

Affiliation Bacteriology Laboratory, Department of P. G. Studies and Research in Biological Science, Rani Durgavati University, Jabalpur, Madhya Pradesh, India

Suresh Kumar

Affiliation Cancer Pharmacology Division, Indian Institute of Integrative Medicine, Jammu, India

Fayaz Malik

Affiliation Cancer Pharmacology Division, Indian Institute of Integrative Medicine, Jammu, India

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: IH AS SK FM. Performed the experiments: IH SK. Analyzed the data: IH. Contributed reagents/materials/analysis tools: IH SK. Wrote the paper: IH.

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Apoptosis
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Enzyme purification
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Cancer treatment
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Cell cycle and cell division
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Cell staining
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Serine proteases
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Asparagine
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Gel electrophoresis
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