Validation of a Multiplex Allele-Specific Polymerase Chain Reaction Assay for Detection of KRAS Gene Mutations in Formalin-Fixed, Paraffin-Embedded Tissues from Colorectal Cancer Patients

Sirirat Seekhuntod; Paninee Thavarungkul; Nuntaree Chaichanawongsaroj

doi:10.1371/journal.pone.0147672

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Open Access

Peer-reviewed

Research Article

Validation of a Multiplex Allele-Specific Polymerase Chain Reaction Assay for Detection of KRAS Gene Mutations in Formalin-Fixed, Paraffin-Embedded Tissues from Colorectal Cancer Patients

Sirirat Seekhuntod,

Affiliation Department of Transfusion Medicine and Clinical Microbiology, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand
⨯
Paninee Thavarungkul,

Affiliation Department of Medical Services, Institute of Pathology, Ministry of Public Health, Bangkok, Thailand
⨯
Nuntaree Chaichanawongsaroj

* E-mail: nuntaree@gmail.com

Affiliation Department of Transfusion Medicine and Clinical Microbiology, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand
⨯

Validation of a Multiplex Allele-Specific Polymerase Chain Reaction Assay for Detection of KRAS Gene Mutations in Formalin-Fixed, Paraffin-Embedded Tissues from Colorectal Cancer Patients

Sirirat Seekhuntod,
Paninee Thavarungkul,
Nuntaree Chaichanawongsaroj

Published: January 26, 2016
https://doi.org/10.1371/journal.pone.0147672

About the Authors

Sirirat Seekhuntod

Affiliation Department of Transfusion Medicine and Clinical Microbiology, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand

Paninee Thavarungkul

Affiliation Department of Medical Services, Institute of Pathology, Ministry of Public Health, Bangkok, Thailand

Nuntaree Chaichanawongsaroj

* E-mail: nuntaree@gmail.com

Affiliation Department of Transfusion Medicine and Clinical Microbiology, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: NC. Performed the experiments: SS. Analyzed the data: SS NC. Contributed reagents/materials/analysis tools: NC PT. Wrote the paper: SS NC.

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Point mutation
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Mutation detection
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Polymerase chain reaction
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Colorectal cancer
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Amplification-refractory mutation system analysis
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DNA cloning
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Mutation
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DNA isolation
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